Integrated SERS-Based Microdroplet Platform for the Automated Immunoassay of F1 Antigens in Yersinia pestis
Integrated SERS-Based Microdroplet Platform for the Automated Immunoassay of F1 Antigens in Yersinia pestis
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DOI：
10.1021/acs.analchem.7b01822
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发表时间：
2017-08-15
影响因子：
7.4
通讯作者：
Choo, Jaebum
中科院分区：
文献类型：
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作者：
Choi, Namhyun;Lee, Jiyoung;Choo, Jaebum
基于表面增强拉曼散射（SERS）的微流控平台的发展最近引起了生物科学界的极大关注。 SERS 是一种高度灵敏的检测方式，与微尺度方法相比，微流控平台具有许多优势，包括高分析通量、简便的自动化和减少的样品需求。因此，SERS 与微流体平台的集成在化学和生物实验中提供了重要的实用性。在此，我们报告了一种完全集成的基于 SERS 的微滴平台，用于鼠疫耶尔森氏菌特异性抗原组分 1 (Fl) 的自动免疫分析。具体来说，通过连续的液滴生成、运输和合并实现高效、快速的免疫反应，同时通过液滴分裂实现免洗免疫检测。这种集成提供了一种新型多功能平台，能够在 nL 体积液滴中进行复杂的多步骤免疫测定。使用基于 SERS 的集成微滴平台检测鼠疫耶尔森氏菌 F1 抗原的限为 59.6 pg/mL，该值比传统酶联免疫吸附测定灵敏约 2 个数量级。本次检测。该系统具有其他优点，包括减少样品消耗（少于 100 id）、快速测定时间（少于 10 分钟）以及全自动流体控制。我们预计，这种基于 SERS 的集成微滴装置将为开发各种有害物质的简便检测平台提供新的见解。
The development of surface-enhanced Raman scattering (SERS)-based microfluidic platforms has attracted significant recent attention in the biological sciences. SERS is a highly sensitive detection modality, with microfluidic platforms providing many advantages over microscale methods, including high analytical throughput, facile automation, and reduced sample requirements. Accordingly, the integration of SERS with microfluidic platforms offers significant utility in chemical and biological experimentation. Herein, we report a fully integrated SERS-based microdroplet platform for the automatic immunoassay of specific antigen fraction 1 (Fl) in Yersinia pestis. Specifically, highly efficient and rapid immunoreactions are achieved through sequential droplet generation, transport, and merging, while wash-free immunodetection is realized through droplet-splitting. Such integration affords a novel multifunctional platforrn capable of performing complex multistep immunoassays in nL-volume droplets. The limit of detection of the F1 antigen for Yersinia pestis using the integrated SERS-based microdroplet platform is 59.6 pg/mL, a value approximately 2 orders of magnitude more sensitive than conventional enzyme-linked immunosorbent assays. This assay. system has additional advantages including reduced sample consumption (less than 100 id), rapid assay times (less than 10 min), and fully automated, fluid control. We anticipate that this integrated SERS-based microdroplet device will provide new insights in the development of facile assay platforms for various hazardous materials.