Cellulose Nanoparticles are a Biodegradable Photoacoustic Contrast Agent for Use in Living Mice.
Cellulose Nanoparticles are a Biodegradable Photoacoustic Contrast Agent for Use in Living Mice.
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DOI：
10.1016/j.pacs.2014.07.001
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发表时间：
2014-09-01
期刊：
影响因子：
7.9
通讯作者：
Gambhir SS
中科院分区：
文献类型：
--
作者：
Jokerst JV;Van de Sompel D;Bohndiek SE;Gambhir SS
光声超声分子成像是一个新兴领域，它将超声的空间和时间分辨率与光学成像的对比度相结合。然而，很少有成像剂，提供高信号强度和生物降解成小分子。在这里，我们描述了一种基于纤维素的纳米颗粒，其峰值光声信号在700 nm处，体外检测限为6 pM（0.02 mg/mL）。使用低至0.35 nM（1.2 mg/mL）的剂量对卵巢癌小鼠模型进行成像。最重要的是，通过葡萄糖测定和电子显微镜，纳米颗粒显示出在纤维素酶存在下的生物降解。
Molecular imaging with photoacoustic ultrasound is an emerging field that combines the spatial and temporal resolution of ultrasound with the contrast of optical imaging. However, there are few imaging agents that offer both high signal intensity and biodegradation into small molecules. Here we describe a cellulose-based nanoparticle with peak photoacoustic signal at 700 nm and an in vitro limit of detection of 6 pM (0.02 mg/mL). Doses down to 0.35 nM (1.2 mg/mL) were used to image mouse models of ovarian cancer. Most importantly, the nanoparticles were shown to biodegrade in the presence of cellulase both through a glucose assay and electron microscopy.