cDNA cloning and genomic structure of human bone morphogenetic protein-3b (BMP-3b)
cDNA cloning and genomic structure of human bone morphogenetic protein-3b (BMP-3b)
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DOI：
10.1006/bbrc.1996.0889
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发表时间：
1996-06-14
影响因子：
3.1
通讯作者：
Kangawa, K
中科院分区：
文献类型：
--
作者：
Hino, J;Takao, M;Kangawa, K
BMP-3b（也称为GDF-10）是最近通过分子克隆在大鼠股骨组织中发现的一种新的BMP-3相关蛋白。本文分离了人BMP-3b的cDNA和基因，并测定了它们的结构。克隆的人BMP-3b cDNA长度为2632个碱基对，编码478个氨基酸的前体蛋白，与大鼠BMP-3b的同源性为83%。人BMP-3b基因由3个外显子组成，跨越大约13个DNA酶。5 '-侧翼区不携带典型的TATA盒，而是富含G+C的序列。Southern印迹分析显示BMP-3b基因位于10号染色体的单个位点。通过北方分析，人BMP-3b转录本主要在股骨、脑、肺、骨骼肌、胰腺和睾丸中检测到。(C)出版社：Academic Press，Inc.
BMP-3b (also termed GDF-10) is a novel BMP-3 related protein recently discovered in rat femur tissue by molecular cloning. In this paper, we have isolated cDNA and the gene for human BMP-3b and determined their structure. Cloned human BMP-3b cDNA with a size of 2632 base pairs encodes a 478 amino acid precursor protein sharing 83% identity with rat BMP-3b. The human BMP-3b gene is composed of 3 exons and spans approximately 13 kilobases of DNA. The 5'-flanking region carries no typical TATA box but G+C rich sequences. Southern blot analysis revealed that the BMP-3b gene is situated in a single locus of chromosome 10. By Northern analysis, human BMP-3b transcripts were detected primarily in femur, brain, lung, skeletal muscle, pancreas and testis. (C) 1996 Academic Press, Inc.