Water-soluble chiral CdSe/CdS dot/rod nanocrystals for two-photon fluorescence lifetime imaging and photodynamic therapy
Water-soluble chiral CdSe/CdS dot/rod nanocrystals for two-photon fluorescence lifetime imaging and photodynamic therapy
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用于双光子荧光寿命成像和光动力治疗的水溶性手性 CdSe/CdS 点/棒纳米晶体
DOI：
10.1039/c9nr04508b
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发表时间：
2019-08-28
期刊：
影响因子：
6.7
通讯作者：
Chen, Rui
中科院分区：
文献类型：
--
作者：
He, Tingchao;Qiu, Xin;Chen, Rui
与传统的有机造影剂相比，半导体纳米晶(NCS)具有独特的光学性质，具有长荧光寿命和大多光子吸收等超高灵敏度的生物学应用。然而，关于手性配体稳定的半导体纳米管的MPA性质及其生物学应用的报道还很少，这严重阻碍了其相关应用。在本工作中，我们报道了利用半胱氨酸分子实现了CdSe/CDS点/棒纳米管的水相转移，并保持了其高的荧光量子产率、长的寿命和高效的圆二色谱。更重要的是，所研究的点/棒纳米管在第一和第二生物窗口显示了极大的双光子和三光子吸收作用截面，在800 nm处的最大值类似于21 000 GM，在1300 nm处的最大值与4.6×10(-78)cm(6)S(2)/光子(2)相似，这是已报道的水溶性荧光纳米颗粒的最大值之一。有趣的是，点状/棒状NCS显示出高达35%的单线态氧产生效率。此外，首次成功地展示了点/棒NCS的双光子荧光寿命成像和光动力学治疗。对这些水溶性的CdSe/CDS点/棒纳米管的光学性质的研究表明，它们在非线性生物成像应用中具有很好的应用前景。
Compared with traditional organic contrast agents, semiconductor nanocrystals (NCs) have unique optical properties that are vital for biological applications with ultrahigh sensitivities, such as long fluorescence lifetime and large multiphoton absorption (MPA). However, the MPA properties and biological applications of chiral-ligand-stabilized semiconductor NCs have scarcely been reported, which seriously hinders their relevant applications. In this work, we report the aqueous phase transfer of CdSe/CdS dot/rod NCs with the use of cysteine molecules, after which the NCs preserve their high fluorescence quantum yield, long lifetime, and efficient circular dichroism. More importantly, the investigated dot/rod NCs show extremely large two- and three-photon absorption action cross-sections in the first and second biological windows, with maximum values of similar to 21 000 GM at 800 nm and similar to 4.6 x 10(-78) cm(6) s(2) per photon(2) at 1300 nm, which are among the largest values reported for water-soluble fluorescent nanoparticles. Interestingly, the dot/rod NCs exhibit a high singlet oxygen generation efficiency of 35%. In addition, for the first time, two-photon fluorescence lifetime imaging and photodynamic therapy of the dot/rod NCs were successfully demonstrated. The performed investigation of the optical properties of these water-soluble CdSe/CdS dot/rod NCs indicates that they are promising candidates for nonlinear biological imaging applications.