Segregation of spermatozoa within sperm storage tubules of fowl and turkey hens
Segregation of spermatozoa within sperm storage tubules of fowl and turkey hens
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DOI：
10.1530/reprod/123.1.79
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发表时间：
2002-01-01
期刊：
影响因子：
3.8
通讯作者：
Donoghue, AA
中科院分区：
文献类型：
--
作者：
King, LA;Brillard, JP;Donoghue, AA
在鸟类物种中，精子在输卵管中被称为精子储存管的特殊结构中长时间驻留，但对不同雄性连续授精后精子在这些小管中的相对分布知之甚少。本研究评价了各种荧光染料对鸡和火鸡精子的染色效果，探讨了一种可能的精子竞争机制。然后分别用染色和未染色的精子在不同的时间间隔进行授精，观察精子在储存小管内的空间分布。几种新型的荧光亲脂示踪剂成功地染色了哺乳动物的精子，它们要么不能染色家禽或火鸡的精子，要么严重损害了精子的活力。相比之下，Hoechst 33342很容易染色精子核(家禽：25 nmol l(-1)；火鸡：77 nmol l(-1))，在4小时内不抑制精子活力，也不影响生育能力或卵子的孵化能力。采用等量染色或未染色的精子，每隔24 h进行连续授精，并在最终授精后24 h处死，研究精子在小管内的进入和储存情况。切除含有精子储存小管的输卵管粘膜，将单个小管分为含有染色精子、未染色精子、染色与未染色精子混合或不含精子。本研究结果表明，在家禽和火鸡中，两种不同授精方式的精子通常会分离到不同的储存管中。仅在4%的家禽和12%的火鸡的储存管中发现含有混合精子的储存管。因此，最后男性优先的机制似乎不是由于小管内精子的分层。
In avian species, spermatozoa reside in the oviduct for prolonged periods in specialized structures known as sperm storage tubules, but little is known about the relative distribution of spermatozoa in these tubules after successive inseminations by different males. The staining efficacies of various fluorescent dyes for fowl and turkey spermatozoa were evaluated to investigate one proposed mechanism of sperm competition. Hens were then inseminated at different intervals with stained and unstained spermatozoa to observe the spatial distribution of spermatozoa within the storage tubules. Several novel fluorescent lipophilic tracers that successfully stain mammalian spermatozoa either did not stain fowl or turkey spermatozoa, or greatly impaired sperm motility. In contrast, Hoechst 33342 readily stained sperm nuclei (fowl: 25 nmol l(-1); turkey: 77 nmol l(-1)) within 4 h without inhibiting sperm motility, or affecting fertility or the hatching ability of the eggs. Hens were tandemly inseminated with equal numbers of stained or unstained spermatozoa at 24 h intervals and were killed 24 h after the final insemination to study sperm entry and storage within the tubules. Oviductal mucosa containing sperm storage tubules was removed, and individual tubules were classified as containing stained spermatozoa, unstained spermatozoa, a mixture of stained and unstained spermatozoa, or as not containing spermatozoa. Results from the present study indicate that spermatozoa from two different inseminations generally segregate into different storage tubules in both fowl and turkey hens. Storage tubules containing mixed populations of spermatozoa were found in only 4% of fowl and 12% of turkey storage tubules examined. Thus, the mechanism of last-male precedence does not appear to be due to the stratification of spermatozoa within the tubules.