Chondroprotective effects of high-molecular-weight cross-linked hyaluronic acid in a rabbit knee osteoarthritis model
Chondroprotective effects of high-molecular-weight cross-linked hyaluronic acid in a rabbit knee osteoarthritis model
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DOI：
10.1016/j.joca.2013.10.005
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发表时间：
2014-01-01
影响因子：
7
通讯作者：
Iwasaki, N.
中科院分区：
文献类型：
--
作者：
Elmorsy, S.;Funakoshi, T.;Iwasaki, N.
目的：我们假设高分子量（MW）交联（CL）透明质酸（HA）可改善关节润滑并具有增强的软骨保护作用。我们检查了注射高分子量 CL HA 后骨关节炎膝关节的组织病理学变化和摩擦系数。设计：使用 20 只日本白兔的双侧前十字韧带横断（ACLT）模型。从横断后第5周开始，每周向10个右膝注射低MW HA (0.8 x 10(6) Da; HA80)或高MW CL HA (6 x 10(6) Da; HA600)，持续3周； 10左膝注射生理盐水(NS)。为了排除五个膝盖的自发性骨关节炎（OA），进行了假手术。通过肉眼观察、组织病理学（菊池评分）、生物力学测试和关节液粘弹性的流变学评估来评估结果。采用单因素方差分析进行统计分析，置信区间 (CI) 为 95%（P < 0.05）。 结果：肉眼观察结果显示，NS 组中 30% 以及 HA80 和 HA600 组中 20% 的软骨严重受损，而假手术组 100% 的软骨完整。 HA600组的组织学评分和摩擦系数显着低于NS组（分别为P=0.007和P=0.002）。关节液的粘弹性测量显示三个治疗组之间没有显着差异。结论：高分子量 CL HA 发挥潜在的软骨保护作用并产生优异的摩擦系数。我们的结果表明，HA600 可以有效延缓 OA 的进展，并显着改善关节润滑。 (C) 2013 年国际骨关节炎研究协会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: We hypothesized that high-molecular-weight (MW) cross-linked (CL) hyaluronic acid (HA) improves joint lubrication and has an enhanced chondroprotective effect. We examined the histopathological changes and friction coefficients in osteoarthritic knee joints after injecting high-MW CL HA.Design: A bilateral anterior cruciate ligament transection (ACLT) model in 20 Japanese white rabbits was used. From week 5 after transection, low-MW HA (0.8 x 10(6) Da; HA80) or high-MW CL HA (6 x 10(6) Da; HA600) was injected weekly into 10 right knee for 3 weeks; normal saline (NS) was injected into the 10 left knee. A sham operation was undertaken to exclude spontaneous osteoarthritis (OA) in five knees. Results were evaluated with macroscopy, histopathology (Kikuchi's score), biomechanical testing, and rheological assessment of the joint fluid viscoelasticity. Statistical analysis was performed using one-way analysis of variance with a 95% confidence interval (CI) (P < 0.05).Results: The macroscopic findings showed severely damaged cartilage in 30% of the NS group and 20% of the HA80 and HA600 groups and intact cartilage in 100% of the sham group. The histological scores and friction coefficients of the HA600 group were significantly lower than those of the NS group (P = 0.007 and P = 0.002, respectively). Viscoelasticity measurements of the joint fluid showed no significant differences between the three treatment groups.Conclusion: High-MW CL HA exerts potential chondroprotective effects and produces superior friction coefficients. Our results suggest that HA600 delays the progression of OA effectively and improves joint lubrication significantly. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.