High efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents
High efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents
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DOI：
10.1016/j.carres.2003.12.007
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发表时间：
2004-02-25
影响因子：
3.1
通讯作者：
Fujita, K
中科院分区：
文献类型：
--
作者：
Akaike, E;Tsutsumida, M;Fujita, K
我们研究了重组冬毛霉内切酶(Endo-M)在有机溶剂中的转糖基化反应。即使在30%的丙酮、二甲基亚砜或甲醇存在下，重组Endo-M也能将蛋黄糖肽中的二唾液双天线复合型低聚糖转糖为对硝基苯基N-乙酰-β-D-氨基葡萄糖苷。转糖基化产物的产率达到受体总量的21-34%，而在水溶液中的产率仅为14%左右。(C)2003爱思唯尔有限公司。保留所有权利。
We examined the transglycosylation reaction by the recombinant endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) expressed in Candida boidinii in media containing organic solvents. The recombinant Endo-M could transglycosylate a disialo biantennary complex-type oligosaccharide from hen egg yolk glycopeptide to p-nitrophenyl N-acetyl-beta-D-glucosaminide even in the presence of 30% acetone, dimethyl sulfoxide, or methanol. The yield of the transglycosylation product reached 21-34% of the total amount of acceptor, while the yield was only about 14% in aqueous solution. (C) 2003 Elsevier Ltd. All rights reserved.