CLASP Facilitates Transitions between Cortical Microtubule Array Patterns
CLASP Facilitates Transitions between Cortical Microtubule Array Patterns
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CLASP 促进皮质微管阵列模式之间的转换
DOI：
10.1104/pp.18.00961
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发表时间：
2018
期刊：
影响因子：
7.4
通讯作者：
Shaw, Sidney L.
中科院分区：
文献类型：
--
作者：
Thoms, David;Vineyard, Laura;Elliott, Andrew;Shaw, Sidney L.
中心体植物微管阵列在细胞皮层形成图案，通过模板化细胞壁材料的沉积来影响细胞形态发生，但微管形成皮层阵列图案的分子基础仍然很大程度上未知。拟南芥 (Arabidopsis thaliana) 微管相关蛋白、细胞质接头相关蛋白 (AtCLASP) 的丢失会导致下胚轴细胞生长各向异性缺陷。我们检查了atclasp-1null突变体中的微管阵列图案，发现阵列图案之间的转换时间存在显着缺陷，但阵列图案本身没有实质性缺陷。对twoatclasp-1荧光微管蛋白标记系中微管动力学的详细分析和计算模型揭示了标记依赖性对解聚和灾难频率的影响，预计会改变稳态微管群。对底层微管阵列架构的定量体内分析表明，在阵列模式之间的转换过程中，需要 AtCLASP 来维持生长的微管正末端的数量。我们认为 AtCLASP 通过对促进阵列转换的新微管的作用在细胞形态发生中发挥关键作用。
Acentrosomal plant microtubule arrays form patterns at the cell cortex that influence cellular morphogenesis by templating the deposition of cell wall materials, but the molecular basis by which the microtubules form the cortical array patterns remains largely unknown. Loss of the Arabidopsis (Arabidopsis thaliana) microtubule-associated protein, CYTOPLASMIC LINKER ASSOCIATED PROTEIN (AtCLASP), results in cellular growth anisotropy defects in hypocotyl cells. We examined the microtubule array patterning inatclasp-1null mutants and discovered a significant defect in the timing of transitions between array patterns but no substantive defect in the array patterns per se. Detailed analysis and computational modeling of the microtubule dynamics in twoatclasp-1fluorescent tubulin marker lines revealed marker-dependent effects on depolymerization and catastrophe frequency predicted to alter the steady-state microtubule population. Quantitative in vivo analysis of the underlying microtubule array architecture showed that AtCLASP is required to maintain the number of growing microtubule plus ends during transitions between array patterns. We propose that AtCLASP plays a critical role in cellular morphogenesis through actions on new microtubules that facilitate array transitions.