Antibiofilm elastin-like polypeptide coatings: functionality, stability, and selectivity
Antibiofilm elastin-like polypeptide coatings: functionality, stability, and selectivity
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DOI：
10.1016/j.actbio.2018.10.039
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发表时间：
2019-01-01
影响因子：
9.7
通讯作者：
Andersson, Martin
中科院分区：
文献类型：
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作者：
Atefyekta, Saba;Pihl, Maria;Andersson, Martin
抗菌肽（AMPS）作为传统抗生素的替代治疗生物材料相关感染的方法目前正受到人们的关注。然而，这些多肽固有的不稳定性往往限制了它们在预期临床应用中的功效。将amp共价固定在表面是提高其长期稳定性和降低其毒性的一种策略。在这项工作中，抗菌肽rrprprprpwwwww - nh2 （RRP9W4N）利用共价化学修饰含有细胞粘附肽结构域（RGD）的弹性蛋白样多肽（ELP）表面涂层。当AMP与ELP表面共价结合时，其对表皮葡萄球菌、金黄色葡萄球菌和铜绿假单胞菌的抗菌活性保持不变。同时，AMP功能化对人MG63骨肉瘤细胞和人间充质干细胞（hMSCs）的活力、功能和分化均无显著影响。此外，研究了固定化AMP在人血清中的稳定性，结果表明AMP的抗菌活性可保持24 h。综合结果表明，共价附着在含rgd的ELP上的AMP是医疗器械抗菌涂层的理想选择。(C) 2018材料学报Elsevier Ltd.出版。版权所有。
Antimicrobial peptides (AMPS) are currently receiving interest as an alternative to conventional antibiotics to treat biomaterial-associated infection. However, the inherent instability of such peptides often limits their efficacy in intended clinical applications. Covalent immobilization of AMPs to surfaces is one strategy to increase the long-term stability and minimize the toxicity. In this work, an antimicrobial peptide, RRPRPRPRPWWWW-NH2 (RRP9W4N), was used to modify elastin-like polypeptide (ELP) surface coatings containing cell-adhesive peptide domains (RGD) using covalent chemistry. The AMP retained its antibacterial activity against Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa when covalently bonded to ELP surfaces. Simultaneously, the AMP functionalization had insignificant effect on the viability, function, and differentiation of human osteosarcoma MG63 cells and human mesenchymal stem cells (hMSCs). Furthermore, stability of the immobilized AMP in human blood serum was investigated, and the results suggested that the AMP preserved its antibacterial activity up to 24 h. Combined, the results show that covalently attached AMPs onto RGD-containing ELP are an excellent candidate as an antimicrobial coating for medical devices. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.