IONIC CURRENTS IN SINGLE SMOOTH-MUSCLE CELLS FROM THE URETER OF THE GUINEA-PIG
IONIC CURRENTS IN SINGLE SMOOTH-MUSCLE CELLS FROM THE URETER OF THE GUINEA-PIG
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DOI：
10.1113/jphysiol.1989.sp017565
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发表时间：
1989-04-01
影响因子：
5.5
通讯作者：
WATANABE, M
中科院分区：
文献类型：
--
作者：
IMAIZUMI, Y;MURAKI, K;WATANABE, M
从酶法分离的豚鼠输尿管平滑肌细胞记录动作电位背后的离子电流。从单细胞记录的动作电位与从多细胞制备的动作电位相似。在第一个峰电位之后的平台电位上显示出重复的峰电位。用10 mM-四乙基铵（TEA）处理增加了平台期的振幅和持续时间，并消除了重复尖峰。动作电位平台期的重复峰电位是由于Ca ~（2+）诱导的Ca ~（2+）释放引起的伊卡的缓慢失活和快速再激活以及IK（Ca）的重复激活所致;（2）动作电位平台期的长时间可能是由于伊卡的缓慢失活、Na ~+依赖的晚期内向电流和小的延迟整流外向电流所致。
Ionic currents underlying the action potential were recorded from enzymatically isolated smooth muscle cells of guinea-pig ureter. The action potential recorded from a single cell was similar to that from a multicellular preparation. It showed repetitive spikes on a plateau potential which followed the first spike. Treatment with 10 mM-tetraethylammonium (TEA) increased the amplitude and duration of the plateau phase and abolished the repetitive spikes. The repetitive spikes on the plateau phase of the action potential are attributable to the slow inactivation and rapid reactivation of ICa and repetitive activation of IK(Ca) due to Ca2+-induced Ca2+ release from stores, and (2) the long plateau phase in the action potential may be due to a slowly inactivating ICa, a Na+-dependent late inward current, and a small delayed rectifier outward current.