New mono-ether of glycerol and triterpenes with DPPH radical scavenging activity from Cameroonian propolis
New mono-ether of glycerol and triterpenes with DPPH radical scavenging activity from Cameroonian propolis
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DOI：
10.1080/14786419.2016.1253077
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发表时间：
2017-02
影响因子：
2.2
通讯作者：
E. Talla;A. N. Tamfu;Isaac Sylvère Gade;Lambert Yanda;J. Mbafor;S. Laurent;L. Elst;M. Popova;V. Bankova
中科院分区：
文献类型：
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作者：
E. Talla;A. N. Tamfu;Isaac Sylvère Gade;Lambert Yanda;J. Mbafor;S. Laurent;L. Elst;M. Popova;V. Bankova
摘要对部分蜂胶样品的提取物进行了GC-MS分析，并用柱层析法进行了纯化。后者分离得到了一个新的甘油单醚1‘-O-二十烷基甘油和一个新的三萜类化合物-3-β，27-二羟基环-24-烯-26-油酸甲酯，以及已知的三个三萜类化合物，即白桦醇，3-β-羟基烷-9，24-二烯-21-酸，芒果酸，氨基酸和β-谷甾醇的混合物，3-β-羟基环-12，24(25)-二烯和27-羟基芒果酸。测定了部分提取物和化合物对DPPH自由基的清除能力。其清除自由基活性从蜂胶正己烷提取物(IC_(50)=5.6 mg/mL)到蜂胶甲醇提取物(IC_(50)=1.07 mg/mL)，从3-β-羟基-9，24-二烯-21-辛酸(IC_(50)=1.22 mg/mL)到1‘-O-二十烷基甘油(IC_(50)=0.93 mg/mL)。样品的活性中等，因为它们接近标准抗氧化剂没食子酸(IC50=0.30 mg/mL)和维生素C(IC50=0.80 mg/mL)，特别是最具活性的化合物1‘-O-二十烷基甘油。
Abstract The extracts of some propolis samples were analysed by GC-MS and then purified by column chromatography. The latter led to the isolation of a new mono-ether of glycerol, 1′-O-eicosanyl glycerol and a new triterpene, methyl-3β,27-dihydroxycycloart-24-en-26-oate together with known triterpenoids namely betulin, 3β-hydroxylanostan-9,24-dien-21-oic acid, mangiferonic acid, a mixture of ambolic acid and β-sitosterol, 3β-hydroxycycloartan-12,24(25)-diene and 27-hydroxymangiferonic acid. The DPPH radical scavenging potential of some extracts and compounds were measured. The radical scavenging activity varied from Hexane extract of Foumban propolis (IC50 = 5.6 mg/mL) to Methanol extract of Foumban propolis (IC50 = 1.07 mg/mL) for the extracts and from 3β-hydroxylanostan-9,24-dien-21-oic acid (IC50 = 1.22 mg/mL) to 1′-O-eicosanyl glycerol (IC50 = 0.93 mg/mL) for the compounds. Activities of samples were moderate as they remained closer to those of the standard antioxidants Gallic acid (IC50 = 0.30 mg/mL) and vitamin C (IC50 = 0.80 mg/mL), especially 1′-O-eicosanyl glycerol, the most active compound.