Genetic linkage mapping of zebrafish genes and ESTs
Genetic linkage mapping of zebrafish genes and ESTs
复制标题
DOI：
10.1101/gr.10.4.558
复制
发表时间：
2000-04-01
期刊：
影响因子：
7
通讯作者：
Talbot, WS
中科院分区：
文献类型：
--
作者：
Kelly, PD;Chu, F;Talbot, WS
斑马鱼（Danio rerio）的通用筛选已经分离出数百种对脊椎动物发育、生理和行为至关重要的基因突变。我们已经构建了一个遗传连锁图谱，这将有助于识别这些突变的候选基因，并允许在斑马鱼和其他脊椎动物的基因组之间进行比较。在这张图谱上，我们定位了771个斑马鱼基因，并通过在减数分裂图谱上记录单链构象多态性（sscp）来表达序列标签（ESTs）。在这些序列中，642个代表以前未定位的基因和ESTs。该图谱由42个单倍体胚胎单细胞期（HS二倍体）经心脏休克处理后产生的纯合子二倍体个体组成。这种“双单倍体”策略结合了单倍体和标准二倍体系统的优势，因为热休克二倍体个体在每个位点只有一个等位基因，并且可以存活到成年，从而可以从作图面板中的每个个体中制备相对大量的基因组DNA。为了整合这张图谱，我们还在图谱面板中记录了593个先前绘制的简单序列长度多态性（slps）。该图谱将加速斑马鱼突变的分子分析，并促进脊椎动物基因组的比较分析。
Generic screens in zebrafish (Danio rerio) have isolated mutations in hundreds of genes essential for vertebrate development, physiology, and behavior. We have constructed a genetic linkage map that will facilitate the identification of candidate genes for these mutations and allow comparisons among the genomes of zebrafish and other vertebrates. On this map, we have localized 771 zebrafish genes and expressed sequence tags (ESTs) by scoring single-stranded conformational polymorphisms (SSCPs) in a meiotic mapping panel. Of these sequences, 642 represent previously unmapped genes and ESTs. The mapping panel was comprised of 42 homozygous diploid individuals produced by hear shock treatment of haploid embryos at the one-cell stage (HS diploids). This "doubled haploid" strategy combines the advantages of mapping in haploid and standard diploid systems, because heat shock diploid individuals have only one allele at each locus and can survive to adulthood, enabling a relatively large quantity of genomic DNA to be prepared from each individual in the mapping panel. To integrate this map with others, we also scored 593 previously mapped simple-sequence length polymorphisms (SSLPs) in the mapping panel. This map will accelerate the molecular analysis of zebrafish mutations and facilitate comparative analysis of vertebrate genomes.