MCM7 Expression Is Altered in Rat After Spinal Cord Injury
MCM7 Expression Is Altered in Rat After Spinal Cord Injury
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脊髓损伤后大鼠 MCM7 表达发生改变
DOI：
10.1007/s12031-013-0003-y
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发表时间：
2013-09-01
影响因子：
3.1
通讯作者：
Cui, Zhiming
中科院分区：
文献类型：
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作者：
Chen, Jiajia;Cui, Zhiming;Cui, Zhiming
微小染色体维持蛋白7(MCM7)是微小染色体维持蛋白家族中的一员，在真核细胞DNA复制起始和延伸过程的早期阶段起着至关重要的作用。MCM7参与了基因组复制的细胞周期调控。虽然MCM7在所有组织中都有表达，但对其在中枢神经系统中的生物学功能仍知之甚少。在本研究中，我们建立了成年大鼠脊髓损伤(SCI)模型。Western blotting显示脊髓损伤后MCM7表达明显改变。免疫组织化学分析显示MCM7在脊髓中广泛分布。双重免疫荧光染色显示脊髓损伤后MCM7免疫反应增强，主要分布于神经元、星形胶质细胞和小胶质细胞。我们还检测了活化的caspase-3、增殖细胞核抗原(PCNA)和Ki67的表达谱，它们的变化与MCM7的表达谱相关。此外，MCM7/活性caspase-3在神经元核(Neun)中有共存，MCM7/PCNA在Neun、胶质纤维酸性蛋白和CD11b中有共存。提示MCM7可能参与了脊髓损伤后神经元的凋亡以及星形胶质细胞和小胶质细胞的增殖。
Minichromosome maintenance protein 7 (MCM7), a member of the minichromosome maintenance protein family, is essential for eukaryotic DNA replication initiation and the early stage of the elongation process. MCM7 participates in the cell cycle control of genome duplication. While it is ubiquitously expressed in all tissues, the biological function of MCM7 in the central nervous system is still with limited acquaintance. In the present study, we performed a spinal cord injury (SCI) model in adult rats. Western blotting indicated a marked alteration of MCM7 after SCI. Immunohistochemistry analysis revealed a wide distribution of MCM7 in the spinal cord. Double immunofluorescence staining showed that MCM7 immunoreactivity was increased predominantly in neurons, astrocytes, and microglia after SCI. We also examined the expression profiles of active caspase-3, proliferating cell nuclear antigen (PCNA), and Ki67, whose changes were correlated with the expression profiles of MCM7. Moreover, colocalization of MCM7/active caspase-3 was detected in neuronal nuclei (NeuN), and colocalization of MCM7/PCNA was detected in NeuN, glial fibrillary acidic protein, and CD11b, respectively. Our results suggest that MCM7 might be implicated in the apoptosis of neuron and proliferation of astrocytes and microglia after SCI.