Interactions between periodontal bacteria and human oral epithelial cells:: Fusobacterium nucleatum adheres to and invades epithelial cells
Interactions between periodontal bacteria and human oral epithelial cells:: Fusobacterium nucleatum adheres to and invades epithelial cells
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DOI：
10.1128/iai.68.6.3140-3146.2000
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发表时间：
2000-06-01
影响因子：
3.1
通讯作者：
Genco, RJ
中科院分区：
文献类型：
--
作者：
Han, YW;Shi, WY;Genco, RJ
细菌是牙周病的病原体。口腔细菌和牙龈上皮细胞之间的相互作用是牙周感染的重要方面。采用体外组织培养模型，检测了一组与牙周病密切相关的革兰氏阴性厌氧菌，包括福赛氏拟杆菌、弯曲弯曲杆菌、腐蚀性艾肯氏杆菌、核梭杆菌、牙龈卟啉单胞菌和中间普雷沃特氏菌的黏附和侵袭人牙周上皮细胞的能力。还检测了这些细菌对促炎趋化因子白介素8(IL-8)的影响。通过对口腔上皮细胞系KB细胞的黏附和侵袭特性进行了检测，并用透射电子显微镜和代谢抑制剂对其侵袭特性进行了验证，发现这种侵袭似乎是通过一种“拉链”机制发生的，需要肌动蛋白、微管、信号转导、蛋白质合成和蛋白质合成参与上皮细胞的蛋白质合成和蛋白质合成。核盘藻的自发突变体LAM，分离自凝集缺陷，不能附着或入侵HGEC或KB细胞，进一步表明在这些过程中需要细菌成分。糖抑制实验表明，核假单胞菌与KB细胞的黏附与凝集素样相互作用有关，对这些新的毒力表型的研究将有助于我们更好地理解核假单胞菌在牙周感染中的作用。
Bacteria are causative agents of periodontal diseases. Interactions between oral bacteria and gingival epithelial cells are essential aspects of periodontal infections. Using an in vitro tissue culture model, a selected group of gram-negative anaerobic bacteria frequently associated Rith periodontal diseases, including Bacteroides forsythus, Campylobacter curvus, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia, were examined for their ability to adhere to and invade primary cultures of human gingival epithelial cells (HGEC). The effects of these bacteria on the production of interleukin-8 (IL-8), a proinflammatory chemokine, were also measured. These studies provided an initial demonstration that F. nucleatum adhered to and invaded HGEC and that this was accompanied by high levels of IL-8 secretion from the epithelial cells, The attachment and invasion characteristics of F. nucleatum were also tested using KB cells, an oral epithelial cell line, The invasion was verified by transmission electron microscopy and with metabolic inhibitors, Invasion appeared to occur via a "zipping" mechanism and required the involvement of actins, microtubules, signal transduction, protein synthesis, and energy metabolism of the epithelial cell, as well as protein synthesis by F. nucleatum. A spontaneous mutant, lam, of F. nucleatum, isolated as defective in autoagglutination, was unable to attach to or invade HGEC or KB cells, further indicating the requirement of bacterial components in these processes. Sugar inhibition assays indicated that lectin-like interactions were involved in the attachment of F. nucleatum to KB cells, Investigation of these new virulence phenotypes should improve our understanding of the role of F. nucleatum in periodontal infections.