Chronic Salmonella infected mouse model.
Chronic Salmonella infected mouse model.
复制标题
DOI：
10.3791/1947
复制
发表时间：
2010-05-31
期刊：
影响因子：
--
通讯作者：
Sun, Jun
中科院分区：
文献类型：
--
作者：
Wu, Shaoping;Lu, Rong;Sun, Jun
细菌感染小鼠模型是研究感染、炎症、免疫学、信号转导和肿瘤发生等领域的强大模型系统。许多研究者利用鼠伤寒沙门氏菌诱导的结肠炎来研究炎症和感染的早期阶段。然而，关于体内慢性感染的报道很少。沙门氏菌在小鼠胃肠道中的持续存在使我们能够探索宿主-细菌的长期相互作用、信号转导和肿瘤发生。我们建立了一个慢性细菌感染的小鼠模型，鼠伤寒沙门氏菌在小鼠肠道定植超过6个月。为了使用该系统，研究人员必须学习如何制备细菌培养物和灌胃动物。我们详细介绍了细菌培养和灌胃小鼠的方法学。我们还展示了如何检测沙门氏菌在胃肠道中的持久性。总的来说，该方案将帮助研究人员使用细菌感染的小鼠模型来解决根本上重要的生物学和微生物学问题。
The bacterial infected mouse model is a powerful model system for studying areas such as infection, inflammation, immunology, signal transduction, and tumorigenesis. Many researchers have taken advantage of the colitis induced by Salmonella typhimurium for the studies on the early phase of inflammation and infection. However, only few reports are on the chronic infection in vivo. Mice with Salmonella persistent existence in the gastrointestinal tract allow us to explore the long-term host-bacterial interaction, signal transduction, and tumorigenesis. We have established a chronic bacterial infected mouse model with Salmonella typhimurium colonization in the mouse intestine over 6 months. To use this system, it is necessary for the researcher to learn how to prepare the bacterial culture and gavage the animals. We detail a methodology for prepare bacterial culture and gavage mice. We also show how to detect the Salmonella persistence in the gastrointestinal tract. Overall, this protocol will aid researchers using the bacterial infected mouse model to address fundamentally important biological and microbiological questions.