Genome-type-specific variation of the 19th intron sequence within the RNA polymerase I largest subunit gene in the genus Oryza
Genome-type-specific variation of the 19th intron sequence within the RNA polymerase I largest subunit gene in the genus Oryza
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DOI：
10.1007/s00606-009-0172-x
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发表时间：
2009-09-01
影响因子：
1.9
通讯作者：
Nakamura, I.
中科院分区：
文献类型：
--
作者：
Takahashi, H.;Sato, T.;Nakamura, I.
水稻PolA1基因编码RNA聚合酶I的最大亚基，全长约15 kb，包含21个外显子，呈现单倍体基因组。稻属共有22个野生种，9种基因组类型：AA、BB、CC、EE、FF、GG、BBCC、CCDD和HHJJ。我们分析了17个水稻品种PolA1基因的第19内含子（PI19）序列。AA种包含2个栽培种，其PI19长度与CC种相近（287 ~ 296 bp）。其中BB （502 bp）和FF （349 bp）的pi19序列较长，EE （217 bp）和GG （222 bp）的pi19序列较短。pi19的大小差异对二倍体（BB和CC）和异源四倍体（BBCC）物种的简单PCR区分特别有用。通过比较7个基因组的PI19序列，推测了它们之间的进化关系。
Rice PolA1 gene, encoding for the largest subunit of RNA polymerase I, spans ca. 15 kb containing 21 exons and presents as a single-copy-per-haploid genome. The genus Oryza comprises 22 wild species and 9 recognized genome types: AA, BB, CC, EE, FF, GG, BBCC, CCDD, and HHJJ. We analyzed sequences of the 19th intron (PI19) within PolA1 genes in 17 Oryza species. The AA species, containing two cultivated species, showed similar length of PI19 to that of CC species (287-296 bp). The longer PI19s were found in BB (502 bp) and FF (349 bp) species, although EE (217 bp) and GG (222 bp) species had shorter sequences. The size differences of the PI19s are particularly useful to discriminate between diploid (BB and CC) and allotetraploid (BBCC) species using simple PCR analysis. The evolutionary relationship among seven genomes was inferred based on the comparison of their PI19 sequences.