Sex differences in blood pro-oxidant status and platelet activation in children admitted with respiratory syncytial virus bronchiolitis: a pilot study
Sex differences in blood pro-oxidant status and platelet activation in children admitted with respiratory syncytial virus bronchiolitis: a pilot study
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DOI：
10.1186/s13052-020-0792-x
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发表时间：
2020-03-06
影响因子：
3.6
通讯作者：
Pietraforte, Donatella
中科院分区：
文献类型：
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作者：
De Jacobis, Isabella Tarissi;Vona, Rosa;Pietraforte, Donatella
背景呼吸道合胞病毒(RSV)是全球儿童毛细支气管炎最常见的病因，也是发展中国家重要的死亡原因。已有研究表明，毛细支气管炎患儿体内氧化剂和抗氧化剂系统之间的平衡被破坏，氧化应激参与了这种疾病的发病。血小板在抗菌宿主防御中发挥着重要作用，并有助于肺血管修复，既是活性氧化物种的靶标，也是活性氧化物种的来源。本研究的主要目的是评估婴儿期呼吸道合胞病毒毛细支气管炎的临床特征和血小板活化的性别差异。方法选择2017年1月至12月在意大利罗马Bambino Gesu儿科医院就诊的毛细支气管炎患者203例，男112例，女91例；此外，在一组被诊断为呼吸道合胞病毒中度毛细支气管炎的患者(15名男孩和12名女孩)中，分别用电子顺磁共振和流式细胞仪进行了氧化应激和血小板特征的初步研究。选择年龄匹配的健康对照组(男10例，女10例)作为对照组。数据分析采用学生t检验、卡方检验和单因素方差分析。结果本研究突出了性别在毛细支气管炎临床病程中的影响。特别是，我们发现：i)男孩的毛细支气管炎的发生率高于女孩(55%比45%)；ii)女孩的C反应蛋白水平高于男孩(分别为1.11 mg/dL和0.92 mg/dL；p<；0.05)；iii)住院期间有不同程度的血小板增多(女孩轻微，男孩严重)。此外，在选定的患者中，我们发现与患有毛细支气管炎的女孩相比，男孩表现出：1)激活的血小板百分比更高(分别为8%和2%；p<；0.05)；3)形成同型聚集体的血小板数量更多(分别为2.36%和0.84%，p<；0.05)。结论毛细支气管炎是一种感染，性别似乎只在临床病程中起调节作用，也应影响治疗的选择。
Background Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in the pediatric population worldwide and an important cause of death in developing countries. It has been demonstrated that the balance between oxidant and antioxidant systems is disrupted in children with bronchiolitis and that oxidative stress contributes to the pathogenesis of this disease. Platelets play an important role in antimicrobial host defenses and contribute to pulmonary vascular repair being either targets or source of reactive oxidizing species. The main purpose of this study was to assessing sex differences in clinical characteristics and platelets activation during RSV bronchiolitis in infancy. Methods In this retrospective study a total of 203 patients (112 boys and 91 girls) with bronchiolitis, aged 12 months or less, admitted to the Bambino Gesu Pediatric Hospital of Rome (Italy) in the period from January to December 2017, were enrolled. Moreover, in a select group of patients (15 boys and 12 girls) with diagnosis of moderate bronchiolitis from RSV, a pilot study on oxidative stress and platelet characteristics was carried out by electron paramagnetic resonance and flow cytometry respectively. Age-matched healthy control subjects (10 boys and 10 girls) were chosen as controls. Data were analyzed using Student' T test, Chi Squared test and one-way ANOVA test. Results This study highlights the influence of sex in the clinical course of bronchiolitis. In particular we found: i) a higher incidence of bronchiolitis in boys than in girls (55% vs 45%); ii) higher C reactive protein values in girls than boys (1.11 mg/dL vs 0.92 mg/dL respectively; p < 0.05); iii) a different degree of thrombocytosis during hospitalization (mild in the girls and severe in the boys). Moreover, in selected patients we found that compared to girls with bronchiolitis, boys showed: i) higher percentage of activated platelets (8% vs 2% respectively; p < 0.05) and iii) higher number of platelets forming homotypic aggregates (2.36% vs 0.84% respectively, p < 0.05). Conclusion The present study affirm that the bronchiolitis is an infection in which sex seems to act as a modulating factor only in the clinical course, influencing also the choice of the therapy should be made.