Generation of colonic IgA-secreting cells in the caecal patch
Generation of colonic IgA-secreting cells in the caecal patch
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DOI：
10.1038/ncomms4704
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发表时间：
2014-04-01
影响因子：
16.6
通讯作者：
Takeda, Kiyoshi
中科院分区：
文献类型：
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作者：
Masahata, Kazunori;Umemoto, Eiji;Takeda, Kiyoshi
肠道相关淋巴组织负责产生免疫球蛋白A分泌细胞。然而，盲肠补片的功能仍不清楚。盲肠补片是阑尾的一种淋巴组织。在这里，我们使用无菌小鼠在阑尾切除术后定植了肠道细菌来分析盲肠补片的作用。阑尾切除的小鼠在定植后，大肠中的IgA(+)细胞延迟积聚，但在小肠中却没有。结肠免疫球蛋白A(+)细胞减少与粪便微生物群组成改变有关。使用可光转换的Kaeed表达或过继转移的小鼠的实验表明，盲肠斑块IgA(+)细胞迁移到大肠和小肠，而Peyer‘s Patch细胞优先招募到小肠。盲肠斑块中的IgA(+)细胞表达较高水平的CCR10。盲肠补片中的树突状细胞，而不是Peyer‘s补片中的树突状细胞，可以诱导共同培养的B细胞上CCR10。因此，盲肠补片是产生IgA分泌细胞的主要部位，这些细胞迁移到大肠。
Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.