Adipokine expression and endothelial function in subclinical hypothyroidism rats.
Adipokine expression and endothelial function in subclinical hypothyroidism rats.
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DOI：
10.1530/ec-18-0007
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发表时间：
2018-03
影响因子：
2.9
通讯作者：
Tian L
中科院分区：
文献类型：
--
作者：
Gong N;Gao C;Chen X;Wang Y;Tian L
本研究的目的是观察亚临床甲状腺功能减退（sHT）大鼠的脂肪因子表达和内皮功能，并确定左旋甲状腺素（LT4）治疗是否影响这些变化。65只雄性Wistar大鼠随机分为5组：对照组；sHT A、B、C组及sHT + T4组。以甲巯咪唑（MMI）诱导sHT大鼠，给药8周后给予LT4治疗。分别用放射免疫法和酶比色法测定甲状腺功能和脂质水平。采用ELISA试剂盒和氮还原法测定血清脂联素（APN）、趋化素（chemerin）、TNF-α、内皮素（ET-1）和一氧化氮（NO）水平。采用RT-PCR和Western blotting检测实验大鼠内脏脂肪组织（VAT）中APN、趋化素和TNF-α的表达。采用苏木精-伊红（HE）染色观察脂肪组织的变化。sHT大鼠体内促甲状腺激素（TSH）、TNF-α、趋化素、ET-1、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平显著高于对照组和sHT + T4大鼠，APN和NO水平显著低于对照组和sHT + T4大鼠。Pearson相关分析显示，趋化素、TNF-α、ET-1、LDL-C、TC、甘油三酯（TG）水平与TSH呈正相关，APN、NO水平与TSH呈负相关。这些发现表明，高TSH水平有助于sHT中脂肪因子和内皮功能障碍的改变，但LT4治疗改善了这些变化。
The purpose of our study was to observe adipokine expression and endothelial function in subclinical hypothyroidism (sHT) rats and to determine whether levothyroxine (LT4) treatment affects these changes. Sixty-five male Wistar rats were randomly divided into five groups: the control group; sHT A, B and C groups and the sHT + T4 group. The sHT rats were induced by methimazole (MMI) and the sHT + T4 rats were administered LT4 treatment after 8 weeks of MMI administration. Thyroid function and lipid levels were measured using radioimmunoassays and enzymatic colorimetric methods, respectively. Serum adiponectin (APN), chemerin, TNF-α, endothelin (ET-1) and nitric oxide (NO) levels were measured using ELISA kits and a nitric-reductive assay. The expression of APN, chemerin and TNF-α in visceral adipose tissue (VAT) was measured in experimental rats using RT-PCR and Western blotting. Hematoxylin–eosin (HE) staining was used to observe changes in adipose tissue. The sHT rats had significantly higher levels of thyroid-stimulating hormone (TSH), TNF-α, chemerin, ET-1, total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) and lower levels of APN and NO than those in control and sHT + T4 rats. Based on Pearson correlation analysis, the levels of chemerin, TNF-α, ET-1, LDL-C, TC and triglyceride (TG) were positively correlated with TSH, but APN and NO levels were negatively correlated with TSH. These findings demonstrated that high TSH levels contribute to the changes of adipokines and endothelial dysfunction in sHT, but LT4 treatment ameliorates those changes.