MiR-9 promotes angiogenesis of endothelial progenitor cell to facilitate thrombi recanalization via targeting TRPM7 through PI3K/Akt/autophagy pathway
MiR-9 promotes angiogenesis of endothelial progenitor cell to facilitate thrombi recanalization via targeting TRPM7 through PI3K/Akt/autophagy pathway
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MiR-9通过PI3K/Akt/自噬途径靶向TRPM7促进内皮祖细胞血管生成促进血栓再通
DOI：
10.1111/jcmm.15124
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发表时间：
2020
影响因子：
5.3
通讯作者：
Li Wen-Dong
中科院分区：
文献类型：
--
作者：
Zhou Dong-Ming;Sun Li-Li;Zhu Jian;Chen Bing;Li Xiao-Qiang;Li Wen-Dong
内皮祖细胞（EPC）已成为血栓再通的有前途的治疗选择。然而，EPC 的这一作用受到一些不利因素的限制。本研究的目的是探讨 miR-9-5p 在调节 EPC 的增殖、迁移和血管生成中的作用以及随后在血栓形成事件中的治疗作用。进行伤口愈合、Transwell实验、管形成实验和体内血管生成实验来分别测量细胞迁移、侵袭和血管生成能力。进行蛋白质印迹以阐明 miR-9-5p 和 TRPM7 在自噬途径中的关系。研究发现miR-9-5p可以通过激活PI3K/Akt/自噬途径减弱TRPM7表达来促进EPCs的迁移、侵袭和血管生成。总之，miR-9-5p 通过 PI3K/Ak/自噬途径靶向 TRPM7，从而介导 EPC 中的细胞增殖、迁移和血管生成。作为潜在的治疗靶点，miR-9-5p 可能在 DVT 的预后中发挥重要作用。
Endothelial progenitor cells (EPCs) have emerged as a promising therapeutic choice for thrombi recanalization. However, this role of EPCs is confined by some detrimental factors. The aim of this study was to explore the role of the miR‐9‐5p in regulation of the proliferation, migration and angiogenesis of EPCs and the subsequent therapeutic role in thrombosis event. Wound healing, transwell assay, tube formation assay and in vivo angiogenesis assay were carried out to measure cell migration, invasion and angiogenic abilities, respectively. Western blot was performed to elucidate the relationship between miR‐9‐5p and TRPM7 in the autophagy pathway. It was found that miR‐9‐5p could promote migration, invasion and angiogenesis of EPCs by attenuating TRPM7 expression via activating PI3K/Akt/autophagy pathway. In conclusion, miR‐9‐5p, targets TRPM7 via the PI3K/Ak/autophagy pathway, thereby mediating cell proliferation, migration and angiogenesis in EPCs. Acting as a potential therapeutic target, miR‐9‐5p may play an important role in the prognosis of DVT.