Therapeutic-designed electrospun bone scaffolds: Mesoporous bioactive nanocarriers in hollow fiber composites to sequentially deliver dual growth factors
Therapeutic-designed electrospun bone scaffolds: Mesoporous bioactive nanocarriers in hollow fiber composites to sequentially deliver dual growth factors
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DOI：
10.1016/j.actbio.2014.12.028
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发表时间：
2015-04-01
影响因子：
9.7
通讯作者：
Kim, Hae-Won
中科院分区：
文献类型：
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作者：
Kang, Min Sil;Kim, Joong-Hyun;Kim, Hae-Won
提出了一种新的治疗设计的纳米纤维支架，持有的能力，负载和提供双生长因子，靶向骨再生。介孔生物活性玻璃纳米球（MBN）被用作生物活性纳米载体，用于成骨增强剂成纤维细胞生长因子18（FGF 18）的长期递送。此外，引入由聚环氧乙烷/聚己内酯制成的生物聚合物纤维的核壳结构，以将FGF 2（另一种类型的细胞增殖和血管生成生长因子）安全地装载在核内，同时比FGF 18更快地释放FGF 2。制备的MBNs显示出约7 nm的扩大的中孔，具有大的表面积和孔体积。当使用细胞色素C（一种模型蛋白质）进行测试时，MBN的蛋白质负载能力高达13%。通过静电纺丝将负载蛋白质的MBN顺利地并入纤维的芯内，同时保持纤维形态。MBN的掺入显着提高了核壳纤维的磷灰石形成能力和力学性能。通过使用细胞色素C证明了将两种实验生长因子FGF 2和FGF 18（掺入核壳纤维（FGF 2）或MBN（FGF 18）内）顺序递送的可能性。使用大鼠间充质干细胞的体外研究证明了FGF 2和FGF 18负载的作用：显著刺激细胞增殖以及诱导碱性磷酸酶活性和细胞矿化。对大鼠颅骨缺损进行6周的体内研究表明，在骨体积和密度方面，加载FGF 2和FGF 18的纤维支架具有显著更高的骨形成能力。目前的设计利用具有核壳结构的新型MBN纳米载体，旨在以顺序方式释放两种类型的生长因子，FGF 2和FGF 18，并被认为提供了一种有前途的治疗支架平台，对骨再生有效。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
A novel therapeutic design of nanofibrous scaffolds, holding a capacity to load and deliver dual growth factors, that targets bone regeneration is proposed. Mesoporous bioactive glass nanospheres (MBNs) were used as bioactive nanocarriers for long-term delivery of the osteogenic enhancer fibroblast growth factor 18 (FGF18). Furthermore, a core shell structure of a biopolymer fiber made of polyethylene oxide/polycaprolactone was introduced to load FGF2, another type of cell proliferative and angiogenic growth factor, safely within the core while releasing it more rapidly than FGF18. The prepared MBNs showed enlarged mesopores of about 7 nm, with a large surface area and pore volume. The protein-loading capacity of MBNs was as high as 13% when tested using cytochrome C, a model protein. The protein-loaded MBNs were smoothly incorporated within the core of the fiber by electrospinning, while preserving a fibrous morphology. The incorporation of MBNs significantly increased the apatite-forming ability and mechanical properties of the core shell fibers. The possibility of sequential delivery of two experimental growth factors, FGF2 and FGF18, incorporated either within the core shell fiber (FGF2) or within MBNs (FGF18), was demonstrated by the use of cytochrome C. In vitro studies using rat mesenchymal stem cells demonstrated the effects of the FGF2 FGF18 loadings: significant stimulation of cell proliferation as well as the induction of alkaline phosphate activity and cellular mineralization. An in vivo study performed on rat calvarium defects for 6 weeks demonstrated that FGF2 FGF18-loaded fiber scaffolds had significantly higher bone-forming ability, in terms of bone volume and density. The current design utilizing novel MBN nanocarriers with a core shell structure aims to release two types of growth factors, FGF2 and FGF18, in a sequential manner, and is considered to provide a promising therapeutic scaffold platform that is effective for bone regeneration. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.