The testing season affects red deer skinfold increase in response to phytohaemagglutinin
The testing season affects red deer skinfold increase in response to phytohaemagglutinin
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DOI：
10.1016/j.prevetmed.2011.02.011
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发表时间：
2011-06-01
影响因子：
2.6
通讯作者：
Gortazar, C.
中科院分区：
文献类型：
--
作者：
Fernandez-de-Mera, I. G.;Jaroso, R.;Gortazar, C.
红鹿（Cervus elaphus）在生理上具有明显的季节性。本研究的目的是评估季节对红鹿对植物血凝素（PHA）有丝分裂原皮试反应的影响。研究对象包括270只人工饲养的成年红鹿（19只公鹿和251只母鹿）。皮肤试验在2009年1月至2010年8月期间进行。动物皮内注射0.1ml体积的含有250 μ g在磷酸盐缓冲盐水中稀释的PHA。在注射前即刻和给药后72 h测量皮褶厚度，始终由同一人进行，每次测量重复三次。性别和时间对皮试反应性的单一影响显著（p < 0.001），它们的交互作用也显著（p < 0.001）。在冬季（1月），并考虑到两年的平均值，皮内注射250 μ g PHA的皮肤褶皱的增加是2.1倍大的雄鹿和1.4倍，在hinds比在夏季（8月）。而雄鹿有1.3倍以上的反应，在冬季，相反发生在夏季，与1.1倍以上的反应，在hinds。我们还证明了有限的年际变化皮褶增加响应PHA在红鹿。这些发现对于解释结核病和副结核病（至少在鹿中）的死前诊断中的皮肤试验结果具有重要意义。(C)2011爱思唯尔有限公司版权所有。
Red deer (Cervus elaphus) have a pronounced seasonality in their physiology. The aim of this study was to assess the effect of the season on red deer responsiveness to skin testing with the phytohaemagglutinin (PHA) mitogen. Study subjects included 270 farmed adult red deer (19 stags and 251 hinds). The skin testing was carried out between January 2009 and August 2010. The animals were injected intradermally with a 0.1 ml volume containing 250 mu g of PHA diluted in phosphate buffered saline. The skinfold thickness was measured immediately prior to injection and 72 h after administration, always by the same person and with three repeats per measurement. Single effects of sex and time on skin test responsiveness were significant (p < 0.001) as well as their interaction (p < 0.001). In winter (January), and considering the average of two years, the skinfold increase in response to the intradermal injection of 250 mu g PHA was 2.1 times larger in stags and 1.4 times in hinds than in summer (August). While stags had 1.3 times larger responses than hinds in winter, the inverse occurred in summer, with 1.1 times larger responses in hinds. We also evidenced a limited inter-annual variation of skinfold increase in response to PHA in red deer. These findings have important consequences regarding the interpretation of skin test results in the ante-mortem diagnosis of tuberculosis and paratuberculosis, at least in deer. (C) 2011 Elsevier B.V. All rights reserved.