Role of the ESAT-6 secretion system in virulence of the emerging community-associated Staphylococcus aureus lineage ST398.
Role of the ESAT-6 secretion system in virulence of the emerging community-associated Staphylococcus aureus lineage ST398.
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ESAT-6 分泌系统在新兴社区相关金黄色葡萄球菌谱系 ST398 毒力中的作用。
DOI：
10.1038/srep25163
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发表时间：
2016-04-26
影响因子：
4.6
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Wang Y;Hu M;Liu Q;Qin J;Dai Y;He L;Li T;Zheng B;Zhou F;Yu K;Fang J;Liu X;Otto M;Li M
新的金黄色葡萄球菌克隆继续出现，在其他健康人群中引起感染。其中一个例子是序列型（ST） 398谱系，我们在这里展示了它作为中国社区相关（CA）人类感染的重要原因的重要性日益增加。我们对是什么决定了这些新出现的克隆具有相当大的毒力潜力，缺乏深刻的理解。关于最近发现的ESAT-6分泌系统（ESS）对毒力的贡献的信息仍然特别少。与主要的医院相关克隆相比，中国ST398分离株表现出显著增加的ESS基因表达，我们发现这可能是由于辅助基因调控（Agr）系统的表达增加以及Agr对ESS的控制。重要的是，ST398中essB的缺失导致小鼠皮肤和血液感染模型中对中性粒细胞杀伤的抵抗力显著降低，毒力降低。我们的研究结果表明，在一种重要的新兴CA-S中，ESS在促进毒力和抵抗先天宿主防御机制方面发挥了关键作用。球菌血统。他们认为，ESS在促进金黄色葡萄球菌的侵袭性毒力和流行病学成功方面的作用迄今被低估了。
Novel Staphylococcus aureus clones continue to emerge that cause infections in otherwise healthy people. One example is the sequence type (ST) 398 lineage, which we show here is increasing in importance as a significant cause of community-associated (CA) human infections in China. We have a profound lack of understanding about what determines the considerable virulence potential of such newly emerging clones. Information about the contribution to virulence of the more recently discovered ESAT-6 secretion system (ESS) has remained particularly scarce. The Chinese ST398 isolates exhibited significantly increased expression of ESS genes as compared to predominant hospital-associated clones, which we found is likely due to increased expression of the accessory gene regulator (Agr) system and control of ESS by Agr. Importantly, deletion of essB in ST398 resulted in significantly reduced resistance to neutrophil killing and decreased virulence in murine skin and blood infection models. Our results demonstrate a key function of ESS in promoting virulence and mechanisms of resistance to innate host defense in an important emerging CA-S. aureus lineage. They suggest that ESS has a so far underestimated role in promoting aggressive virulence and epidemiological success of S. aureus.