Identification and expression analysis of the DREB transcription factor family in pineapple (Ananas comosus (L.) Merr.)
Identification and expression analysis of the DREB transcription factor family in pineapple (Ananas comosus (L.) Merr.)
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DOI：
10.7717/peerj.9006
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发表时间：
2020-04-28
期刊：
影响因子：
2.7
通讯作者：
Qin, Yuan
中科院分区：
文献类型：
--
作者：
Chai, Mengnan;Cheng, Han;Qin, Yuan
背景：脱水反应元件结合(DREB)转录因子在植物的生长发育和逆境反应中起着至关重要的作用。虽然DREB基因在许多植物中已被鉴定，但在菠萝(Ananas comosus(L.)结果：通过全基因组筛选，我们在14条染色体上鉴定出20个AcoDREB基因。这些人被分成五个亚组。同一组内的AcoDREB具有相似的基因结构和结构域组成。通过基因结构分析，我们发现大多数AcoDREB基因(75%)缺乏内含子，并且所有20个AcoDREB基因的启动子区域至少有一个与胁迫反应相关的顺式元件。我们在所有分析的组织中发现了四个高表达的基因和六个低表达的基因。我们检测了8个AcoDREB基因在非生物胁迫下的表达变化。结论：本报告首次对菠萝DREB转录因子家族进行了全基因组分析。我们的研究结果为今后菠萝AcoDREB基因的功能分析提供了初步数据，也为培育具有抗逆性等关键农艺性状的菠萝新品种提供了有用的信息。
Background: Dehydration responsive element-binding (DREB) transcription factors play a crucial role in plant growth, development and stress responses. Although DREB genes have been characterized in many plant species, genome-wide identification of the DREB gene family has not yet been reported in pineapple (Ananas comosus (L.) Merr.).Results: Using comprehensive genome-wide screening, we identified 20 AcoDREB genes on 14 chromosomes. These were categorized into five subgroups. AcoDREBs within a group had similar gene structures and domain compositions. Using gene structure analysis, we showed that most AcoDREB genes (75%) lacked introns, and that the promoter regions of all 20 AcoDREB genes had at least one stress response-related cis-element. We identified four genes with high expression levels and six genes with low expression levels in all analyzed tissues. We detected expression changes under abiotic stress for eight selected AcoDREB genes.Conclusions: This report presents the first genome-wide analysis of the DREB transcription factor family in pineapple. Our results provide preliminary data for future functional analysis of AcoDREB genes in pineapple, and useful information for developing new pineapple varieties with key agronomic traits such as stress tolerance.