Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
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尿糖胺聚糖与绝经后女性复发性尿路感染和尿生物组生态有关。
DOI：
10.1101/2023.01.11.523678
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
DeNisco,NicoleJ
中科院分区：
文献类型：
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作者：
Neugent,MichaelL;Hulyalkar,NehaV;Kumar,Ashwani;Xing,Chao;Zimmern,PhilippeE;Shulaev,Vladimir;DeNisco,NicoleJ
糖胺聚糖 (GAG) 是线性带负电荷的多糖，由糖醛酸和氨基糖的重复二糖单元组成。膀胱上皮的管腔表面覆盖有 GAG 层。这些尿路上皮 GAG 被认为提供了保护屏障，并作为与泌尿微生物组 (urobiome) 的潜在相互作用位点。先前的研究已经分析了混合队列中尿 GAG 的组成，但绝经后妇女的尿 GAG 组成仍不清楚。为了研究 GAG 与复发性尿路感染 (rUTI) 之间的关系，我们对绝经后女性对照队列中的尿 GAG 进行了分析。我们发现硫酸软骨素 (CS) 是绝经后女性主要的尿 GAG，并且患有活动性 rUTI 的女性尿 CS 升高。我们还将尿 GAG 与尿生物组组成相关联，并鉴定了与尿 GAG 浓度显着相关的细菌种类。 无枝杆菌、桑毛单胞菌和巴氏葡萄球菌与硫酸肝素或透明质酸呈正相关，与阴道菌群失调相关的细菌种类与尿 CS 呈负相关。总而言之，这项工作定义了与 rUTI 相关的尿 GAG 组成的变化，并确定了尿 GAG 与尿生物组之间可能在 rUTI 病理学中发挥作用的新关联。
Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars. The luminal surface of the bladder epithelium is coated with a GAG layer. These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome). Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined. To investigate the relationship between GAGs and recurrent urinary tract infection (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women. We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI. We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.Corynebacterium amycolatum,Porphyromonas somerae, andStaphylococcus pasteuriwere positively associated with heparin sulfate or hyaluronic acid, and bacterial species associated with vaginal dysbiosis were negatively correlated with urinary CS. Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.