Stoichiometry of the reactions of calcium with the metallochromic indicator dyes antipyrylazo III and arsenazo III.
Stoichiometry of the reactions of calcium with the metallochromic indicator dyes antipyrylazo III and arsenazo III.
复制标题
钙与金属显色指示剂染料安替吡唑 III 和胂 III 反应的化学计量。
DOI：
10.1016/s0006-3495(81)84755-8
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发表时间：
1981
影响因子：
3.4
通讯作者：
Schneider,MF
中科院分区：
文献类型：
--
作者：
Ríos,E;Schneider,MF
提出了一种测定金属离子与有机配体的单产物反应的化学计量比的方法，并应用于钙和镁与金属显色染料安替比林偶氮III和偶氮胂III的反应。该方法包括拟合滴定数据，获得的缓冲溶液中的金属，与理论功能，包括：（a）产品浓度对两种反应物的浓度的依赖性，（B）金属离子浓度和总量之间的关系，在缓冲液的存在下加入，和（c）的金属离子的量，结合到有机配体的校正。结果表明，偶氮安替比林Ⅲ与钙、镁反应的产物分别为CaD_2和MgD。在[Ca2+]超过20 μ M时，钙和偶氮胂III之间形成的产物是CaD2，其他产物在[Ca2+]较低时积累。安替比林偶氮III：钙反应的动力学是快速的条件下，这种染料已被应用于测量骨骼肌纤维中的钙瞬变。目前的结果提供了一个校准与安替比林偶氮III在肌纤维的先前研究。
A method for determining the stoichiometry of one-product reactions involving a metal ion and an organic ligand is presented and applied to the reactions of calcium and magnesium with the metallochromic dyes Antipyrylazo III and Arsenazo III. The method consists of fitting titration data, obtained in solutions buffered for the metal, with theoretical functions that include: (a) the dependence of product concentration on the concentration of both reactants, (b) the relationship between metal ion concentration and total amount added in the presence of the buffer, and (c) a correction for the amount of metal ion that binds to the organic ligand. It is shown that the products of the reactions of Antipyrylazo III with calcium and magnesium are CaD2 and MgD, respectively. The product formed between calcium and Arsenazo III at [Ca2+] over 20 microM is CaD2, other products accumulating at lower [Ca2+]. The kinetics of the Antipyrylazo III:Ca reaction are rapid under conditions in which this dye has been applied to measure calcium transients in skeletal muscle fibers. The present results provide a calibration for previous studies with Antipyrylazo III in muscle fibers.