STRUCTURAL AND GERMINATION DEFECTS OF BACILLUS-SUBTILIS SPORES WITH ALTERED CONTENTS OF A SPORE COAT PROTEIN
STRUCTURAL AND GERMINATION DEFECTS OF BACILLUS-SUBTILIS SPORES WITH ALTERED CONTENTS OF A SPORE COAT PROTEIN
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DOI：
10.1128/jb.173.20.6618-6625.1991
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发表时间：
1991-10-01
影响因子：
3.2
通讯作者：
ARONSON, AI
中科院分区：
文献类型：
--
作者：
BOURNE, N;FITZJAMES, PC;ARONSON, AI
测定了枯草芽孢杆菌孢子外壳蛋白基因cotT的转录和翻译起始位点。以10.1 kDa的前体合成CotT蛋白，并将其加工成7.8 kDa的被膜多肽。cotT基因的插入失活导致孢子内层外表发生改变，并且在含有果糖、葡萄糖和天冬酰胺的萌发液中萌发缓慢。在l -丙氨酸和Penassay培养基中的发芽率与野生型相同。在低拷贝数质粒上含有cotT基因的菌株产生的孢子具有过量的cotT前体和增厚的条纹内层。这些孢子对上面提到的所有生发体的反应都很差。在CotT加工位点的密码子位点定向突变导致孢子细胞和孢子上的前体积累，但对孢子的发芽率和耐溶剂性没有影响。科特的缺乏和过量生产都导致孢子内被毛结构和孢子对特定生发物的反应能力发生微妙的变化。
The start sites for transcription and translation of a Bacillus subtilis spore coat protein gene, cotT, were determined. The CotT protein was synthesized as a 10.1-kDa precursor which was processed to a coat polypeptide of 7.8 kDa. Insertional inactivation of the cotT gene resulted in spores with an altered appearance of the inner coat layers and slow germination in response to a germination solution containing fructose, glucose, and asparagine. Rates of germination in L-alanine and in Penassay broth were the same as that of the wild type. A strain containing the cotT gene on a low-copy-number plasmid produced spores with an excess of CotT precursor and a thickening of the striated inner coat. These spores responded poorly to all of the germinants mentioned above. A site-directed mutation of the codon at the processing site of CotT resulted in the accumulation of the precursor in sporulating cells and on the spores, but there was no effect on the germination rates or solvent resistance of these spores. Both the lack and the overproduction of CotT led to subtle alterations in the structure of the inner spore coat and in the capacity of spores to respond to particular germinants.