In vitro activity of nonoxynol-9 on McCoy cells infected with Chlamydia trachomatis.
In vitro activity of nonoxynol-9 on McCoy cells infected with Chlamydia trachomatis.
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nonoxynol-9 对沙眼衣原体感染的 McCoy 细胞的体外活性。
DOI：
10.1097/00007435-198707000-00009
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发表时间：
1987
影响因子：
3.1
通讯作者：
Wyrick,PB
中科院分区：
文献类型：
--
作者：
Knight,ST;Lee,SH;Davis,CH;Moorman,DR;Hodinka,RL;Wyrick,PB
壬醇-9是一种非离子洗涤剂，也是杀精子避孕药的活性成分，已有报道具有抗衣原体活性。然而，在本研究中，沙眼衣原体血清基本体暴露于非氧合酚-9(12.5-10,000μg/ml)对衣原体感染性没有影响。相比之下，未感染的McCoy细胞在72小时内与浓度增加的非氧合酶-9孵育后显示出与剂量相关的细胞毒性。当感染的McCoy细胞暴露于九酚-9时，发育中的衣原体包涵体即使在数量和外观上与未暴露的感染单层中的衣原体包涵体相似，也不会被碘染色。经非氧合酚处理的感染细胞的透射电子显微镜显示内含膜和网状小体明显受损。子代阴性碘包涵体中衣原体的感染度仅为0.3%。我们的结论是，非氧合酶-9的主要作用是在McCoy细胞上，可能对细胞内寄生虫有继发性作用。
Nonoxynol-9, a nonionic detergent and active ingredient in spermicidal contraceptives, has been reported to have antichlamydial properties. However, in this study exposure of elementary bodies of Chlamydia trachomatis serovar to nonoxynol-9 (12.5-10,000 μg/ml) had no effect on chlamydial infectivity. In contrast, uninfected McCoy cells incubated with increasing concentrations of nonoxynol-9 over 72 hr displayed dose-related cytotoxicity. When infected McCoy cells were exposed to nonoxynol-9, the developing chlamydial inclusions did not stain with iodine even though they were similar in number and appearance to the inclusions in unexposed, infected monolayers. Transmission electron microscopy of nonoxynol-treated, infected cells revealed apparent damage to the inclusion membrane and reticulate bodies within. The infectivity of the chlamydiae in the iodine-negative inclusions on subpassage was only 0.3%. We conclude that the primary action of nonoxynol-9 is on the McCoy cell and that there may be secondary effects on the intracellular parasite.