Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum.
Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum.
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DOI：
10.3390/ijms17091541
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发表时间：
2016-09-13
影响因子：
5.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Li P;Wu Z;Liu T;Wang Y
研究了花椒内生真菌的生物多样性、发生规律和抑菌活性。共获得940个分离物，分为93个形态型，43种，23属，根据rDNA内转录间隔区（ITS）序列分析的分子鉴定。结果表明，Z. bungeanum的物种丰富度S（43）、Margalef指数D '（6.1351）、Shannon-Wiener指数H'（3.2743）、Simpson多样性指数Ds（0.9476）、PIE指数（0.9486）和均匀度Pielou指数J（0.8705）较高，但优势度指数λ（0.0524）较低。结果表明，内生真菌在Z.而物种丰富度指数和多样性指数均以茎部最高。采用邻接法对93个内生菌株进行系统发育分析，以揭示其进化过程。结果表明，8株内生真菌对寄主病原真菌接骨木镰刀菌（Fusarium sambucinum）和花椒假尾孢菌（Pseudocercospora zanthropii）均表现出较强的抑制作用。在这里，我们第一次系统地展示了与Z.本研究揭示了从植物不同组织中获取最大的内生真菌物种多样性的价值，为进一步研究和利用内生真菌作为一种独特的生物活性物质提供了框架。
This study investigated the biodiversity, phylogeny, and antifungal activity of endophytic fungi isolated from Zanthoxylum bungeanum. A total of 940 isolates obtained were grouped into 93 morphotypes, 43 species, and 23 genera, which were authenticated by molecular identification based on rDNA internal transcribed spacer (ITS) sequence analysis. A high diversity of endophytic fungi from Z. bungeanum are observed with high species richness S (43), Margalef index D′ (6.1351), Shannon–Wiener index H′ (3.2743), Simpson diversity index Ds (0.9476), PIE index (0.9486), and evenness Pielou index J (0.8705) but a low dominant index λ (0.0524). Significant tissue specificity of the endophytic fungi was observed in Z. bungeanum, and the highest species richness and diversity indexes were obtained in the stem. Phylogenetic analyses of the 93 endophytic isolates were carried out by the neighbor-joining (NJ) method to demonstrate their evolutionary processes. Antifungal activities of endophytic fungi were assayed and eight endophytic isolates showed strong and long-lasting inhibition against host pathogenic fungi Fusarium sambucinum and Pseudocercospora zanthoxyli. Here, for the first time, we systematically demonstrate the biodiversity, phylogeny, and antifungal activity of endophytic fungi associated with Z. bungeanum and reveal the value of sampling different tissues of a given plant to obtain the greatest endophyte species diversity, which might offer a framework for further investigation and utilization of endophytic fungi as aunique source of interesting and useful bioactive compounds.
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