Application of Fe3O4 mesoporous sphere modified carbon ionic liquid electrode as electrochemical hemoglobin biosensor
Application of Fe3O4 mesoporous sphere modified carbon ionic liquid electrode as electrochemical hemoglobin biosensor
复制标题
Fe3O4介孔球修饰碳离子液体电极作为电化学血红蛋白生物传感器的应用
DOI：
10.1016/j.colsurfb.2012.06.010
复制
发表时间：
2013-01-01
影响因子：
5.8
通讯作者：
Wang, Mei
中科院分区：
文献类型：
--
作者：
Sun, Wei;Sun, Zhaolan;Wang, Mei
基于Fe3O4介孔球修饰碳离子液体电极（CILE）构建了一种新型血红蛋白（Hb）电化学生物传感器，该电极对三氯乙酸的还原表现出优异的电催化能力。以六氟磷酸n -己基吡啶（HPPF6）为碳糊的改性剂和粘结剂制备了CILE。紫外可见光谱和傅里叶变换红外光谱结果表明，Hb分子在壳聚糖和Fe3O4介孔球复合膜中保持了原有的结构。电化学结果表明，在0.1 mol/L的磷酸盐缓冲溶液（PBS）中出现了一对清晰的氧化还原峰，形成电位（E-0′）为-0.287 V (vs. SCE)，表明复合膜实现了Hb的直接电子转移。研究了Hb的电化学行为，计算了电子传递系数（alpha）、电子传递数(n)和非均相电子传递速率常数（k(s)）等电化学参数。由于Fe3O4介孔球存在于电极表面的特殊特性，极大地促进了Hb的电子转移速率。Hb修饰电极具有较宽的线性范围和较低的检出限等优良的电催化性能。(C) 2012 Elsevier B.V.版权所有
A new electrochemical hemoglobin (Hb) biosensor was constructed based on a Fe3O4 mesoporous spheres modified carbon ionic liquid electrode (CILE), which showed excellent electrocatalytic ability towards the reduction of trichloroacetic acid. CILE was prepared by using N-hexylpyridinium hexafluorophosphate (HPPF6) as the modifier and the binder in the carbon paste. Ultraviolet-visible and Fourier transform infrared spectroscopic results indicated that Hb molecules retained the native structure in the chitosan and Fe3O4 mesoporous spheres composite film. Electrochemical results indicated that a pair of well-defined redox peaks appeared in 0.1 mol/L phosphate buffer solution (PBS) with the formed potential (E-0') as -0.287 V (vs. SCE), indicating that the direct electron transfer of Hb in the composite film was realized. Electrochemical behaviors of Hb were carefully investigated with the electrochemical parameters such as electron transfer coefficient (alpha), electron transfer number (n), and heterogeneous electron transfer rate constant (k(s)) calculated. Due to the specific characteristics of Fe3O4 mesoporous spheres present on the electrode surface, the electron transfer rate of Hb was greatly promoted. The Hb modified electrode exhibited excellent electrocatalytic properties with wider linear range and lower detection limit. (C) 2012 Elsevier B.V. All rights reserved.