Gibberellins, jasmonate and abscisic acid modulate the sucrose-induced expression of anthocyanin biosynthetic genes in Arabidopsis
Gibberellins, jasmonate and abscisic acid modulate the sucrose-induced expression of anthocyanin biosynthetic genes in Arabidopsis
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DOI：
10.1111/j.1469-8137.2008.02511.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Perata, Pierdomenico
中科院分区：
文献类型：
--
作者：
Loreti, Elena;Povero, Giovanni;Perata, Pierdomenico
花青素是植物次生代谢产物，在植物生理中起着重要作用。蔗糖和激素都能调节花青素的合成。本研究在拟南芥幼苗中研究了蔗糖调控花青素生物合成酶基因的表达，以及蔗糖与植物激素之间的相互作用。采用实时逆转录聚合酶链式反应（RT-PCR）技术，分析了蔗糖和植物激素处理下拟南芥幼苗花青素生物合成途径14个相关基因（包括2个转录因子PAP1、PAP2）的表达谱。蔗糖对花青素合成途径的诱导作用受赤霉素酸（GA）的抑制，而茉莉酸（JA）和脱落酸（ABA）与蔗糖有协同作用。gai突变体对ga依赖的二氢黄酮醇还原酶抑制不太敏感。这似乎证明了GAI信号参与了野生型拟南芥幼苗中蔗糖和GA之间的串扰。相反，蔗糖的诱导作用并不是严格由ABA介导的。蔗糖诱导花青素基因需要COI1基因，而不需要JAR1基因，这表明茉莉酸盐和蔗糖信号通路可能是趋同的。结果表明，在花青素生物合成途径的调控中，存在蔗糖和激素信号通路之间的串扰。
Anthocyanins are secondary metabolites, which play an important role in the physiology of plants. Both sucrose and hormones regulate anthocyanin synthesis. Here, the interplay between sucrose and plant hormones was investigated in the expression of sucrose-regulated genes coding for anthocyanin biosynthetic enzymes in Arabidopsis seedlings.The expression pattern of 14 genes involved in the anthocyanin biosynthetic pathway, including two transcription factors (PAP1, PAP2), was analysed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) in Arabidopsis seedlings treated with sucrose and plant hormones.Sucrose-induction of the anthocyanin synthesis pathway was repressed by the addition of gibberellic acid (GA) whereas jasmonate (JA) and abscisic acid (ABA) had a synergic effect with sucrose. The gai mutant was less sensitive to GA-dependent repression of dihydroflavonol reductase. This would seem to prove that GAI signalling is involved in the crosstalk between sucrose and GA in wild-type Arabidopsis seedlings. Conversely, the inductive effect of sucrose was not strictly ABA mediated. Sucrose induction of anthocyanin genes required the COI1 gene, but not JAR1, which suggests a possible convergence of the jasmonate- and sucrose-signalling pathways.The results suggest the existence of a crosstalk between the sucrose and hormone signalling pathways in the regulation of the anthocyanin biosynthetic pathway.