AMP-activated protein kinase contributes to myofibrillar protein hydrolysis in bovine skeletal muscle through postmortem mitochondrial dysfunction-induced apoptosis.
AMP-activated protein kinase contributes to myofibrillar protein hydrolysis in bovine skeletal muscle through postmortem mitochondrial dysfunction-induced apoptosis.
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DOI：
10.1111/jfbc.14028
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发表时间：
2021-12
影响因子：
4
通讯作者：
Jibing Ma;Cheng Chen;Qunli Yu;Ling Han
中科院分区：
文献类型：
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作者：
Jibing Ma;Cheng Chen;Qunli Yu;Ling Han
本研究旨在验证 AMP 激活蛋白激酶 (AMPK) 在线粒体功能障碍诱导的细胞凋亡和死后牛肌肉嫩化中的作用。在 168 小时老化期间，评估了对照组和化合物 C（AMPK 抑制剂）组的 AMPK 磷酸化水平、线粒体功能障碍、线粒体凋亡因子和肌原纤维蛋白水解。与化合物C组相比，对照组在6-120小时时AMPK活性极其显着增加（p < .01），并且在6和12小时时线粒体Bax/Bcl-2比率分别升高62.3%和42.1%（p < .05）。此外，对照组在 6-72 小时和 12-72 小时分别具有显着或极显着较高的线粒体功能障碍和细胞质细胞色素 c 含量 (p < .05，p < .01)； a caspase-3 表达水平在 12、24 和 72 小时分别增加 23.2%、26.5% 和 26.1% (p < .05)； 24-168 小时时凋亡细胞核的比例显着升高 (p < .05)； 168 小时时，45-、38-、36-、30-和 28-kDa 蛋白质的产量分别增加了 30.8%、35.8%、43.9% 和 39.5% (p < .05)。综上所述，这些结果表明，激活的 AMPK 通过增加线粒体膜上的 Bax/Bcl-2 比率，促进死后衰老过程中的线粒体凋亡和牛肌肉嫩化。实际应用：基于消费者的喜好，冰鲜鲜肉正逐渐成为肉类行业的未来趋势。嫩化不良的牛肉往往会影响消费者的二次购买欲望，给肉类行业带来巨大损失。因此，调节死后线粒体凋亡和牛肌肉嫩化的 AMP 激活蛋白激酶是一个有效的研究目标。
This study aimed to verify the role of AMP-activated protein kinase (AMPK) in mitochondrial dysfunction-induced apoptosis and postmortem bovine muscle tenderization. AMPK phosphorylation levels, mitochondrial dysfunction, mitochondrial apoptotic factors, and myofibrillar protein hydrolysis were assessed in the control group and Compound C (AMPK inhibitor) group over a 168 hr aging period. Compared with the Compound C group, the control group had an extremely significantly increased AMPK activity at 6-120 hr (p < .01) and a 62.3% and 42.1% higher mitochondrial Bax/Bcl-2 ratio at 6 and 12 hr, respectively (p < .05). Moreover, the control group had a significantly or extremely significantly higher mitochondrial dysfunction and cytoplasmic cytochrome c content at 6-72 and 12-72 hr, respectively (p < .05, p < .01); a 23.2%, 26.5%, and 26.1% increased caspase-3 expression levels at 12, 24, and 72 hr, respectively (p < .05); a significantly higher proportion of apoptotic nuclei at 24-168 hr (p < .05); and a 30.8%, 35.8%, 43.9%, and 39.5% increased production of 45-, 38-, 36-, 30-, and 28-kDa proteins at 168 hr, respectively (p < .05). Taken together, these results suggested that activated AMPK promoted mitochondrial apoptosis and bovine muscle tenderization during postmortem aging by increasing the Bax/Bcl-2 ratio on the mitochondrial membrane. PRACTICAL APPLICATIONS: Based on consumer preference, chilled fresh meat is gradually becoming the future trend of the meat industry. Poorly tenderized beef often affects consumers' desire to make secondary purchases and leads to large losses to the meat industry. Therefore, AMP-activated protein kinase, which regulates postmortem mitochondrial apoptosis and bovine muscle tenderization, is a valid research target.