Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction
Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction
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DOI：
10.1007/978-1-60327-029-8_13
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发表时间：
2010-01-01
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影响因子：
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通讯作者：
Kadowitz, Philip J.
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文献类型：
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作者：
Deng, W.;Bivalacqua, T. J.;Kadowitz, Philip J.
勃起功能障碍（ED）被定义为无法达到和/或维持足以令人满意的性行为的阴茎勃起。氧化应激已被证明与年龄或糖尿病相关性ED的病理生理有关。超氧化物歧化酶（SOD）是一种催化超氧化物阴离子（O-2（中心点-））转化为过氧化氢（H2O2）和分子氧（O-2）的抗氧化酶。体内基因治疗和基于成人stein细胞的离体基因治疗是目前治疗ED的两种有吸引力的基因治疗方法。在本章中，我们描述了两种有效的基因转移技术，将治疗基因细胞外超氧化物歧化酶（ecSOD）输送到老年或糖尿病大鼠的阴茎中治疗ED：腺病毒介导的海绵体内ecSOD基因转移用于ED和ecSOD基因修饰的骨髓基质细胞的基因治疗，也称为间充质干细胞，基于干细胞和基因治疗。
Erectile dysfunction (ED) is defined as the inability to attain and/or maintain penile erection sufficient for satisfactory sexual performance. Oxidative stress has been demonstrated to be involved in the pathophysiology of age- or diabetes-related ED. Superoxide dismutase (SOD), an antioxidant enzyme catalyzing the conversion Of Superoxide anion (O-2(center dot-)) to hydrogen peroxide (H2O2) and molecular oxygen (O-2), is a promising therapeutic target for ED. In vivo gene therapy and adult stein cell-based ex vivo gene therapy are two attractive Current gene therapies for the treatment of ED. In this chapter we describe the use of two potent gene transfer techniques to deliver the therapeutic gene extracellular Superoxide dismutase (ecSOD) into the penis of aged or diabetic rats for therapy of ED: adenoviral-mediated intracavernosal ecSOD gene transfer for gene therapy of ED and ecSOD gene-modified marrow stromal cells, also known as mesenchymal stein cells, based stem cell and gene therapy.