Synergistic anticancer activity of photo- and chemoresponsive nanoformulation based on polylysine-functionalized graphene
Synergistic anticancer activity of photo- and chemoresponsive nanoformulation based on polylysine-functionalized graphene
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基于聚赖氨酸功能化石墨烯的光和化学响应纳米制剂的协同抗癌活性
DOI：
10.1021/am5066128
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发表时间：
2014
影响因子：
9.5
通讯作者：
Liu Yiyao
中科院分区：
文献类型：
--
作者：
Wu Chunhui;He Qiuming;Zhu Anni;Li Dan;Xu Min;Yang Hong;Liu Yiyao
基于纳米材料的癌症联合治疗多模式治疗剂引起了越来越多的关注。在本报告中，通过在生物相容性聚-L-赖氨酸（PLL）接枝石墨烯（G-PLL）上组装光敏剂锌（II）-酞菁（ZnPc）和抗癌药物阿霉素（DOX），制备了一种新型光和化学活性纳米杂化物。这种 G-PLL/DOX/ZnPc 纳米复合物表现出优异的理化性质，包括在生物溶液中的高溶解度和稳定性、高载药效率、pH 触发药物释放以及光激发下泛化 1O2 的能力。与游离药物分子相比，用 G-PLL/DOX/ZnPc 处理的细胞表现出更高的细胞摄取。特别是，G-PLL/DOX/ZnPc 由于光动力和化疗效应的结合而产生了显着的协同抗癌活性。组合剂量减少指数显示，DOX 与 ZnPc 组合对三种测试的癌细胞系（HeLa、MCF-7 和 B16）具有很强的协同作用（组合指数 < 0.1）。因此，本研究证明了以 G-PLL/DOX/ZnPc 为代表的可编程双模疗法可协同治疗癌症。
Multimodal therapeutic agents based on nanomaterials for cancer combination therapy have attracted increasing attention. In this report, a novel photo- and chemoactive nanohybrid was fabricated by assembling photosensitizer Zn(II)–phthalocyanine (ZnPc) and anticancer drug doxorubicin (DOX) on the biocompatible poly-l-lysine (PLL)-grafted graphene (G-PLL). This nanocomplex of G-PLL/DOX/ZnPc showed excellent physiochemical properties, including high solubility and stability in biological solutions, high drug loading efficiency, pH-triggered drug release, and ability to generalize1O2under light excitation. Compared to free drug molecules, cells treated with G-PLL/DOX/ZnPc showed a higher cellular uptake. In particular, G-PLL/DOX/ZnPc elicited a remarkable synergistic anticancer activity owing to combined photodynamic and chemotherapeutic effects. The combination dose reduction indexes revealed that combining DOX with ZnPc provided strong synergistic effects (combination index < 0.1) against three cancer cell lines tested (HeLa, MCF-7, and B16). Thus, this study demonstrates programmable dual-modality therapy exemplified by G-PLL/DOX/ZnPc to synergistically treat cancers.