Genomic structure, chromosome location, and alternative splicing of the human NKG2A gene
Genomic structure, chromosome location, and alternative splicing of the human NKG2A gene
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DOI：
10.1007/bf02602558
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发表时间：
1996-01-01
期刊：
影响因子：
3.2
通讯作者：
Trowsdale, J
中科院分区：
文献类型：
--
作者：
Plougastel, B;Jones, T;Trowsdale, J
分离到一株含有人类NKG2A基因的cosmid。对该基因进行了部分测序，发现了7个外显子，包括一个5'未翻译外显子。已发表的cDNA克隆NKG2B缺失54碱基对外显子5，与差异剪接的存在一致。正常淋巴细胞总RNA的逆转录聚合酶链反应证实了这一点。通过FISH将NKG2A基因定位到12p12.3-p13.1染色体上，靠近CD69和Prp基因。这些数据支持人类凝集素样NK基因复合物的存在，类似于小鼠6号染色体上的NK复合物。
A cosmid containing the human NKG2A gene was isolated. The gene was partially sequenced, revealing 7 exons, including one 5' untranslated exon. The 54 base pair exon 5 was missing in the published cDNA clone NKG2B, consistent with the existence of differential splicing. This was confirmed by reverse transcription-polymerase chain reaction of total RNA from normal lymphocytes. The NKG2A gene was mapped by FISH to chromosome 12p12.3-p13.1 in proximity to the CD69 and Prp genes. These data support the presence of a human lectin-like NK gene complex, analogous to the NK complex on mouse chromosome 6.