Physical characteristics of proteins of the electron transfer system and interpretation of the structure of the mitochondrion.
Physical characteristics of proteins of the electron transfer system and interpretation of the structure of the mitochondrion.
复制标题
电子传递系统蛋白质的物理特性和线粒体结构的解释。
DOI：
10.1021/bi00911a015
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发表时间：
1962
期刊：
影响因子：
2.9
通讯作者：
H. Tisdale
中科院分区：
文献类型：
--
作者：
R. Criddle;R. Bock;D. E. Green;H. Tisdale
从牛心线粒体中大量分离出一种蛋白质，它满足结构蛋白的各种标准。通过超速离心、电泳和末端基团分析确定了分离的结构蛋白的同质性。在中性pH下，结构蛋白形成水不溶性聚合物聚集体，但在pH 11或存在阴离子去污剂的情况下，可以证明蛋白质的单体形式（mw 2 × 10 × 104）。细胞色素a、B和Ci在聚合物-单体转变方面表现出类似的行为。结构蛋白质与每种细胞色素形成一对一的水溶性复合物，这些复合物在pH 7的水性介质中可溶;这些复合物的身份已被严格确定。疏水键是聚合现象和结构蛋白和细胞色素单体之间形成复合物的主要类型。结构蛋白能够结合磷脂，这一特性为三种细胞色素所共有。结构蛋白和细胞色素之间以及结构蛋白和脂质之间的相互作用与线粒体组织问题有相当大的关系。多年来人们认识到，分子氧对柠檬酸循环底物的氧化依赖于构成多酶系统的催化单元的结构完整性（绿色，1956-1957）。几个观察结果导致了这样的假设，即参与这种氧化的电子转移发生在一系列氧化还原酶中，这些酶不仅在酶的意义上而且在结构意义上被组织起来。酶的有组织的系统这一事实本身就暗示了一种复合物，在这种复合物中，分子水平上的特定化学相互作用使分子阵列稳定。因此，对线粒体蛋白质的研究可以提供有关有组织酶系统的结构和
A protein has been isolated in largeamounts from beef heart mitochondria which satisfies various criteria for a structural protein. The homogeneity of the isolated structural protein has been established by ultracentrifugal, electrophoretic, and end-group anal-ysis. At neutral pH the structural protein forms a water-insoluble polymeric aggregate, but at pH 11, or in the presence of anionic detergents, a monomeric form of the protein can be demonstrated (mw 2 X 10X 104). Cytochromes a, b, and Ci show analogous behavior with respect to the polymer-monomer transition. Structural pro-tein forms one-to-one water-soluble complexes with each of the cytochromes, which are soluble in aqueous media at pH 7; the identity of these complexes has been rigorously established. The hydrophobic bond is thepredominant type responsible both for the polymerization phenomenon and for complex formation between the monomeric species of the structural protein and of the cytochromes. Structural protein is capable of binding phospholipid; this property is shared by the three cytochromes. The interactions between structuralprotein and cytochromes and between structural protein and lipid have considerable relevance to the problem of mitochondrial organization.For many years it has been recognized that the oxidation of substrates of the citric acid cycle by molecular oxygen depends on the structural in-tegrity of a catalytic unit constituting a multi-enzyme system (Green, 1956-1957). Several ob-servations have ledto the postulate that the transfer of electrons involved in this oxidation takes place within an array of oxidation-reduction enzymes that are organized not only in an enzy-matic sense but also in a structural sense. The very fact of an organized system of enzymes sug-gests a complex in which specific chemical inter-actions at the molecular level stabilize the molec-ular array. The study of mitochondrial proteins can, therefore, yield information on both the structure of the organized enzyme system and
从牛心线粒体中分离出高活性 H -ATP 酶。
影响因子：
3
作者：
Hughes,J;Joshi,S;Torok,K;Sanadi,DR
通讯作者：
Sanadi,DR