HOMOLOGY BETWEEN PHENYLALANINE AND TYROSINE HYDROXYLASES REVEALS COMMON STRUCTURAL AND FUNCTIONAL DOMAINS
HOMOLOGY BETWEEN PHENYLALANINE AND TYROSINE HYDROXYLASES REVEALS COMMON STRUCTURAL AND FUNCTIONAL DOMAINS
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DOI：
10.1021/bi00335a001
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
WOO, SLC
中科院分区：
文献类型：
--
作者：
LEDLEY, FD;DILELLA, AG;WOO, SLC
苯丙氨酸羟基酶(PAH)和酪氨酸羟基酶(TyH)是一类具有多种生物化学和免疫学特性的混合功能氧化酶。最近克隆和测序了人PAH和大鼠TYH的全长cDNA，从而可以详细比较它们的一级结构。多环芳烃和酪氨酸在核酸和氨基酸水平上有很高的同源性。同源性模式表明，这些分子是由包含酶活性决定因素的同源核心和有助于底物特异性和调节的非同源区域组成的。同源性程度也表明这两种蛋白质是从一个共同的祖先进化而来的。
Phenylalanine hydroxylase (PAH) and tyrosine hydroxylase (TYH) are mixed-function oxidases that share many characteristic biochemical and immunological properties. The recent cloning and sequencing of full-length cDNA for both human PAH and rat TYH allow detailed comparison of their primary structures. There is a high degree of homology between PAH and TYH on nucleic acid and amino acid levels. The pattern of homology suggests that these molecules are comparised of a homologous core containing the determinants of enzymatic activity and a nonhomologous region that contributes to substrate specificity and regulation. The degree of homology also suggests that these 2 proteins evolved from a common ancestor.