Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion.
Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion.
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DOI：
10.1152/ajpregu.00482.2005
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发表时间：
2006-05
期刊：
影响因子：
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通讯作者：
F. Katoh;M. Tresguerres;Kyung Mi Lee;T. Kaneko;K. Aida;G. Goss
中科院分区：
文献类型：
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作者：
F. Katoh;M. Tresguerres;Kyung Mi Lee;T. Kaneko;K. Aida;G. Goss
肾脏在淡水鱼和海水鱼的离子调节中起着重要作用。然而，人们对硬骨鱼肾脏中的离子转运机制知之甚少，特别是在分子水平上。我们从虹鳟鱼 (Oncorhynchus mykiss) 中克隆了肾脏特异性的 SLC26 硫酸盐/阴离子交换剂，它与哺乳动物 SLC26A1 (Sat-1) 同源。注射 Na2SO4 后过量血浆硫酸盐浓度的排泄对应于克隆的 SLC26A1 mRNA 的显着较高表达。还通过光学显微镜和透射电子显微镜对虹鳟鱼肾小管进行了详细的形态学观察。根据细胞质中刷状缘和管状系统的结构，虹鳟鱼的肾小管分为近端小管第一段和第二段（PI和PII）和远端小管。原位杂交表明，SLC26A1 阴离子交换剂 mRNA 特异性定位于适应海水和淡水的虹鳟鱼肾脏的 PI 段。免疫细胞化学显示，Na+-K+-ATP酶和液泡型H+-ATP酶共定位于同一细胞，并分别分布在SLC26A1 mRNA表达的细胞的基底膜和顶膜中。这些发现表明，克隆的肾脏特异性 SLC26A1 位于肾近端小管中，并参与虹鳟鱼过量血浆硫酸盐的排泄。
The kidney plays an important role in ion regulation in both freshwater and seawater fish. However, ion transport mechanisms in the teleost kidney are poorly understood, especially at the molecular level. We have cloned a kidney-specific SLC26 sulfate/anion exchanger from rainbow trout (Oncorhynchus mykiss) that is homologous to the mammalian SLC26A1 (Sat-1). Excretion of excess plasma sulfate concentration after Na2SO4 injection corresponded to significantly higher expression of the cloned SLC26A1 mRNA. Detailed morphological observation of rainbow trout renal tubules was also performed by light microscopy and transmission electron microscopy. According to the structure of brush border and tubular system in the cytoplasm, renal tubules of rainbow trout were classified into proximal tubule first and second (PI and PII) segments and distal tubules. In situ hybridization revealed that SLC26A1 anion exchanger mRNA is specifically localized in the PI segment of kidneys from both seawater- and freshwater-adapted rainbow trout. With immunocytochemistry, Na+-K+-ATPase and vacuolar-type H+-ATPase were colocalized to the same cells and distributed in the basolateral and the apical membranes, respectively, of the cells where the SLC26A1 mRNA expressed. These findings suggest that the cloned kidney-specific SLC26A1 is located in kidney proximal tubules and is involved in excretion of excess plasma sulfate in rainbow trout.