IDENTIFICATION AND CHARACTERIZATION OF CTX-M-15 PRODUCING KLEBSIELLA PNEUMONIAE CLONE ST101 IN A HUNGARIAN UNIVERSITY TEACHING HOSPITAL
IDENTIFICATION AND CHARACTERIZATION OF CTX-M-15 PRODUCING KLEBSIELLA PNEUMONIAE CLONE ST101 IN A HUNGARIAN UNIVERSITY TEACHING HOSPITAL
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DOI：
10.1556/030.62.2015.3.2
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发表时间：
2015-09-01
影响因子：
1.5
通讯作者：
Tigyi, Zoltan
中科院分区：
文献类型：
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作者：
Melegh, Szilvia;Schneider, Gyoergy;Tigyi, Zoltan
我们调查了2004-2008年匈牙利佩奇大学教学医院产超广谱β-内酰胺酶（ESBL）肺炎克雷伯菌分离株的分子流行病学。对102株分离株进行分子分型、药敏试验、常见β-内酰胺酶基因（bla（CTX-M）、bla（TEM）和bla（SHV））检测和毒力相关性状（高粘滞性、magA、k2 a、rmpA、铁载体、1型和3型菌毛、生物膜形成、血清耐药性）检测。结果显示存在三种主要的环丙沙星抗性CTX-M-15产生克隆（ST 15 n = 69、ST 101 n = 10和ST 147 n = 9），其中ST 15占优势并且普遍分布。考虑到时间和地点的分布，与ST 15相比，ST 101和ST 147在更少的住院单位和更窄的时间范围内被检测到。除主要克隆外，还鉴定了11个次要克隆，并显示具有以下β-内酰胺酶基因：6个克隆携带bla（CTX-M），4个克隆携带bla（SHV）-5，1个克隆同时具有bla（CTX-M）和ESBL型bla（SHV）。在SHV-5生产的K. pneumoniae克隆中发现了一种新的序列类型，即ST 1193，其具有独特的infB等位基因。不同的毒力因子含量和独特的抗菌药物敏感性谱的特点，每个克隆。与对环丙沙星表现出高水平耐药性的主要克隆分离株相比，次要克隆分离株对环丙沙星表现出显著较低的MIC值，表明氟喹诺酮类在主要K. pneumoniae克隆。这是首次描述CTX-M-15产生K. pneumoniae clone ST 101在匈牙利的研究。
We investigated the molecular epidemiology of extended spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae isolates derived from the teaching hospitals of University of Pecs, Pecs, Hungary in the time period 2004-2008. Molecular typing, antimicrobial susceptibility testing, detection of common beta-lactamase genes (bla(CTX-M), bla(TEM) and bla(SHV)) and virulence associated traits (hypermucoviscosity, magA, k2a, rmpA, siderophores, type 1 and 3 fimbria, biofilm formation, serum resistance) were performed for 102 isolates. The results showed the presence of three major ciprofloxacin resistant CTX-M-15 producing clones (ST15 n = 69, ST101 n = 10, and ST147 n = 9), of which ST15 was predominant and universally widespread. Considering distribution in time and place, ST101 and ST147 were detected at fewer inpatient units and within a narrower time frame, as compared to ST15. Beside major clones, eleven minor clones were identified, and were shown to harbour the following beta-lactamase genes: six clones carried bla(CTX-M), four clones harboured bla(SHV)-5 and one clone possessed both bla(CTX-M) and ESBL type bla(SHV). Among the SHV-5 producing K. pneumoniae clones a novel sequence type was found, namely ST1193, which harboured a unique infB allele. Different virulence factor content and peculiar antimicrobial susceptibility profile were characteristic for each clone. In contrast to major clone isolates, which showed high level resistance to ciprofloxacin, minor clone isolates displayed significantly lower MIC values for ciprofloxacin suggesting a role for fluoroquinolones in the dissemination of the major K. pneumoniae clones. This is the first description of the CTX-M-15 producing K. pneumoniae clone ST101 in Hungary.