Characterization of a Transitional Preplasmablast Population in the Process of Human B Cell to Plasma Cell Differentiation
Characterization of a Transitional Preplasmablast Population in the Process of Human B Cell to Plasma Cell Differentiation
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DOI：
10.4049/jimmunol.1101230
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发表时间：
2011-10-15
影响因子：
4.4
通讯作者：
Klein, Bernard
中科院分区：
文献类型：
--
作者：
Jourdan, Michel;Caraux, Anouk;Klein, Bernard
人B细胞分化为浆细胞的早期步骤知之甚少。我们报道了在体外人记忆B细胞向浆细胞分化的过程中，CD20（低/-）CD38（-）前浆母细胞沿着分化的过渡群体。前浆母细胞缺乏记录的B细胞或浆细胞（CD 20、CD 38和CD 138）标记物，表达CD 30和IL-6 R，并以比浆母细胞或浆细胞更弱的水平分泌Ig。这些前浆母细胞进一步分化为CD 20（-）CD 38（高）CD 138（-）浆母细胞，然后分化为CD 20（-）CD 38（高）CD 138（+）浆细胞。前浆母细胞在全基因组转录组谱和表型方面得到充分表征。前浆母细胞共表达B和浆细胞转录因子，但与B细胞、浆母细胞或浆细胞相比水平降低。它们主要表达未剪接形式的XBP1 mRNA，而浆母细胞和浆细胞基本上表达剪接形式。在人淋巴结（0.06%的CD19（+）细胞）和扁桃体（0.05%的CD19（+）细胞）中可检测到体外生成的前浆母细胞的体内对应物（CD19（+）CD20（低/-）CD38（-）IL-6R（+）细胞）。本发明提供了一种开放存取"B to Plasma Cell Atlas"，其使得可以询问B细胞向浆细胞分化过程中的基因表达。两者合计，我们的研究结果表明，存在一个过渡preplasmablast人口使用浆细胞生成的体外模型，其在体内对应的各种淋巴组织。免疫学杂志，2011，187：3931 - 3941。
The early steps of differentiation of human B cells into plasma cells are poorly known. We report a transitional population of CD20(low/-) CD38(-) preplasmablasts along differentiation of human memory B cells into plasma cells in vitro. Preplasmablasts lack documented B cell or plasma cell (CD20, CD38, and CD138) markers, express CD30 and IL-6R, and secrete Igs at a weaker level than do plasmablasts or plasma cells. These preplasmablasts further differentiate into CD20(-)CD38(high)CD138(-) plasmablasts and then CD20(-)CD38(high)CD138(+) plasma cells. Preplasmablasts were fully characterized in terms of whole genome transcriptome profiling and phenotype. Preplasmablasts coexpress B and plasma cell transcription factors, but at a reduced level compared with B cells, plasmablasts, or plasma cells. They express the unspliced form of XBP1 mRNA mainly, whereas plasmablasts and plasma cells express essentially the spliced form. An in vivo counterpart (CD19(+)CD20(low/-)CD38(-)IL-6R(+) cells) of in vitro-generated preplasmablasts could be detected in human lymph nodes (0.06% of CD19(+) cells) and tonsils (0.05% of CD19(+) cells). An open access "B to Plasma Cell Atlas," which makes it possible to interrogate gene expression in the process of B cell to plasma cell differentiation, is provided. Taken together, our findings show the existence of a transitional preplasmablast population using an in vitro model of plasma cell generation and of its in vivo counterpart in various lymphoid tissues. The Journal of Immunology, 2011, 187: 3931-3941.