In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells
In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells
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DOI：
10.1002/jbm.a.30357
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发表时间：
2005-09-01
影响因子：
4.9
通讯作者：
Fernandes, MH
中科院分区：
文献类型：
--
作者：
Dias, AG;Lopes, MA;Fernandes, MH
在CaO-P2O5-MgO体系中制备了两种含有K2O或TiO2氧化物的玻璃陶瓷，目的是将它们用作潜在的骨移植替代品。TiO2和K2O的掺入使得玻璃陶瓷在结构上形成了特定的晶相，表现出不同程度的生物降解。事实上，45CaO-45P(2)O(5)-5MgO-5K(2)O在水溶液中比45CaO-37P(2)O(5)-5MgO-13TiO(2)玻璃陶瓷更容易溶解。利用人骨髓成骨细胞培养28 d，研究两种磷酸钙玻璃陶瓷的体外生物活性，并评估种子材料的细胞增殖和功能。从宏观和SEM观察来看，含钛玻璃陶瓷在整个培养过程中表现出稳定的表面。成骨细胞逐渐增殖，特别是在第三周，碱性磷酸酶活性高，形成矿化基质。扫描电镜观察，附着细胞呈典型的成骨细胞的扩展多边形形态，细胞间广泛接触。在种子材料上的细胞行为与在组织培养级聚苯乙烯上的细胞行为相似；除了前两周细胞数量较少。相比之下，含k玻璃陶瓷表现出高度不稳定的表面，溶解/沉淀过程在整个培养时间内发生。很少有细胞粘附在材料表面，随后的增殖也受到阻碍，特别是从第一周开始。在大部分孵育时间内，细胞数量明显低于含钛玻璃陶瓷中观察到的细胞数量。结果表明，这两种玻璃陶瓷的体外生物学行为的不同主要是由于表面降解速率的显著差异，而表面降解速率的显著差异与母玻璃的化学成分直接相关。(c) 2005 Wiley期刊有限公司
Two glass ceramics in the CaO-P2O5-MgO System with the incorporation of K2O or TiO2 oxides were prepared with the goal of using them as potential bone graft substitutes. The incorporation of TiO2 and K2O led to the preparation of specific crystalline phases in the structure of the glass ceramics, which show different degrees of biodegradation. In fact, the 45CaO-45P(2)O(5)-5MgO-5K(2)O has been previously demonstrated to be much more soluble in aqueous solutions than the 45CaO-37P(2)O(5)-5MgO-13TiO(2) glass ceramic. The in vitro biological activity of the two calcium phosphate glass ceramics was studied with the use of human bone marrow osteoblast cell cultures maintained for 28 days, and seeded materials were assessed for cell proliferation and function. The Ti-containing glass ceramic showed a stable surface throughout the culture time, on macroscopic and SEM observation. Osteoblast cells proliferated gradually, especially during the third week, with a high alkaline phosphatase activity and formation of a mineralized matrix. On SEM observation, attached cells appeared with a spread-polygonal morphology typical of the osteoblast cells, with extensive cell-to-cell contact. Cell behavior on the seeded material was similar to that found on cultures performed on tissue-culture-grade polystyrene; except for the presence of lower cell numbers during the first 2 weeks. By contrast, the K-containing glass ceramic showed a highly instable surface with dissolution/precipitation processes occurring throughout the culture time. Few cells adhered to the material surface, and subsequent proliferation was also hindered, especially from the first week onwards. Cell numbers were significantly lower than those observed in the Ti-containing glass ceramic during most of the incubation time. Results suggest that the different in vitro biological behavior of these two glass ceramics is mainly due to the significant differences in the surface degradation rate, which is directly correlated to the chemical composition of the mother glass. (c) 2005 Wiley Periodicals, Inc.