Transformation by Rous sarcoma virus: effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides.
Transformation by Rous sarcoma virus: effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides.
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DOI：
10.1073/pnas.76.10.5212
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发表时间：
1979-10
期刊：
影响因子：
--
通讯作者：
K. Radke;G. Martin
中科院分区：
文献类型：
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作者：
K. Radke;G. Martin
摘要劳斯肉瘤病毒感染鸡胚成纤维细胞后，引起细胞生长和形态的多种改变。我们已经使用二维聚丙烯酰胺凝胶电泳来检查病毒转化对细胞多肽的合成和磷酸化模式的影响。劳斯肉瘤病毒感染似乎不会诱导从头合成，或完全抑制，任何[35 S]甲硫氨酸标记的细胞多肽，可以用这种技术解决，但是，有一个小部分（约4%）的[35 S]甲硫氨酸标记的多肽的定量变化。当细胞标记的[32 P]正磷酸盐进行检查，磷酸化的多肽，先生36,000，被检测到在转化的细胞，这种多肽出现在20分钟内，当细胞感染的劳斯肉瘤病毒的温度敏感突变体从非允许的允许温度。因此，36，000 Mr多肽的磷酸化代表转化过程中的早期事件，并且该多肽可能是与pp 60 src相关的激酶活性的靶标。
Abstract Infection of chicken embryo fibroblasts by Rous sarcoma virus induces a variety of alterations in cellular growth and morphology. We have used two-dimensional polyacrylamide gel electrophoresis to examine the effects of viral transformation on the pattern of synthesis and phosphorylation of cellular polypeptides. Infection by Rous sarcoma virus does not appear to induce the de novo synthesis, or the complete suppression, of any of the [35S]methionine-labeled cellular polypeptides that can be resolved with this technique; however, there are quantitative changes in a minor fraction (approximately 4%) of the [35S]methionine-labeled polypeptides. When cells labeled with [32P]orthophosphate were examined, a phosphorylated polypeptide, Mr 36,000, was detected in transformed cells; this polypeptide appears within 20 min when cells infected by a temperature-sensitive mutant of Rous sarcoma virus are shifted from the nonpermissive to the permissive temperature. Phosphorylation of the 36,000 Mr polypeptide thus represents an early event in the process of transformation, and it is possible that this polypeptide is a target for the kinase activity associated with pp60src.