A cellular and spatial map of the choroid plexus across brain ventricles and ages.
A cellular and spatial map of the choroid plexus across brain ventricles and ages.
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DOI：
10.1016/j.cell.2021.04.003
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发表时间：
2021-05-27
期刊：
影响因子：
64.5
通讯作者：
Lehtinen MK
中科院分区：
文献类型：
--
作者：
Dani N;Herbst RH;McCabe C;Green GS;Kaiser K;Head JP;Cui J;Shipley FB;Jang A;Dionne D;Nguyen L;Rodman C;Riesenfeld SJ;Prochazka J;Prochazkova M;Sedlacek R;Zhang F;Bryja V;Rozenblatt-Rosen O;Habib N;Regev A;Lehtinen MK
每个脑室中的脉络丛（ChP）产生脑脊液（CSF）并形成血-CSF屏障。在这里，我们构建了一个单细胞和空间图谱的每个ChP在发展中，成年和老年小鼠大脑。我们描绘了不同年龄和心室的上皮细胞和间充质细胞的不同细胞类型，亚型，细胞状态和表达程序。在发展中的中国药典，我们预测一个共同的祖细胞池的上皮细胞和神经元细胞，谱系追踪验证。上皮细胞和成纤维细胞显示心室区域化表达，从胚胎阶段开始并随着年龄的增长而持续，随着成熟而发生戏剧性的转录转变，并且在每种老年细胞类型中发生较小的转变。随着衰老，上皮细胞上调宿主防御程序，驻留的巨噬细胞上调IL-1β信号转导基因。我们的图谱揭示了ChP脑屏障发育、成熟和老化过程中心室的细胞多样性、结构和信号传导。
The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells showed regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulated host defense programs and resident macrophages upregulated IL-1β signaling genes. Our atlas revealed cellular diversity, architecture and signaling across ventricles during development, maturation and aging of the ChP brain barrier.