Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization
Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization
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中肋骨条藻对 ATP 利用的转录组和生理反应
DOI：
10.1111/1462-2920.14944
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发表时间：
2020
影响因子：
5.1
通讯作者：
Liu Dongyan
中科院分区：
文献类型：
--
作者：
Zhang Xiaohua;Lin Senjie;Liu Dongyan
当水体中溶解性无机磷（DIP）的有效性较低时，浮游植物利用溶解性有机磷（DOP）的能力在其资源竞争中起着重要作用。在这里，我们探讨了全球分布的海洋硅藻，中肋骨条藻的生理和分子响应，利用腺苷-5 ′-三磷酸（ATP）的基础上孵育实验在ATP，DIP-充满，和DIP-耗尽的条件。结果表明，ATP对S. costatumas efficiently as DIP. S.中肋骨条肌参与ATP的利用，但与碱性磷酸酶（AP）无关，AP是一种重要的DOP水解酶，但参与胞外水解。转录组分析揭示了与水解酶活性相关的几种转录物（例如NAD+二磷酸酶），其在ATP培养组中显著上调，表明它们可能参与ATP水解。与此同时，ATP增长。中肋骨条肌与一系列代谢活动（如嘌呤代谢）相关的基因表达下调，显然是为了适应ATP条件。
The capacity of phytoplankton to utilize dissolved organic phosphorus (DOP) plays an important role in their competition for resources when the availability of dissolved inorganic phosphorus (DIP) is low in the aquatic systems. Here, we explored the physiological and molecular responses of a globally distributed marine diatom,Skeletonema costatum, in utilizing adenosine‐5′‐triphosphate (ATP) based on incubation experiments under ATP, DIP‐replete, and DIP‐depleted conditions. The results show that ATP supports the growth ofS. costatumas efficiently as DIP. The pathway ofS. costatuminvolved in utilizing ATP is not related to alkaline phosphatase (AP), an important DOP hydrolase, although extracellular hydrolysis is involved. The transcriptome analysis revealed several transcripts related to the hydrolase activity (e.g. NAD+ diphosphatase), which were significantly upregulated in the ATP culture group, indicating their possible involvement in ATP hydrolysis. Meanwhile, ATP‐grownS. costatumexhibited downregulation of the genes related to a series of metabolic activities (e.g. purine metabolism), apparently to adapt to ATP condition.