Ginsenoside 20(S)-protopanaxadiol inhibits the proliferation and invasion of human fibrosarcoma HT1080 cells
Ginsenoside 20(S)-protopanaxadiol inhibits the proliferation and invasion of human fibrosarcoma HT1080 cells
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DOI：
10.1111/j.1742-7843.2006.pto_415.x
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发表时间：
2006-06-01
影响因子：
3.1
通讯作者：
Wang, Ziren
中科院分区：
文献类型：
--
作者：
Li, Gang;Wang, Zhenhua;Wang, Ziren
人参皂苷20(S)-原人参二醇是人参皂苷的代谢产物之一，在多种癌细胞中具有多效抗癌作用。本研究旨在探讨人参皂苷20(S)-原人参二醇在无细胞毒性的情况下对人纤维肉瘤HT1080细胞体外侵袭及基质金属蛋白酶-2表达的影响。结果表明，人参皂苷20(S)-原人参二醇对HT1080细胞的增殖有浓度依赖性的抑制作用（IC50为76.78 +/- 2.24 μ M， 48小时）。体外侵袭实验中，20(S)-原嘌呤二醇显著降低HT1080细胞的侵袭能力（P < 0.01）。明胶酶谱和western blotting分析显示基质金属蛋白酶-2活性和表达量呈浓度依赖性显著降低（P < 0.01）。综上所述，人参皂苷20(S)-protopanaxadiol能够在体外显著抑制HT1080细胞的侵袭性，其作用可能与下调基质金属蛋白酶-2的表达有关。人参皂苷20(S)-原人参二醇是人参的代谢物，在预防和治疗癌症方面具有潜在的治疗价值。
Ginsenoside 20(S)-protopanaxadiol, one of metabolites of ginseng saponins, has been well characterized to possess the pleiotropic anticancer capabilities in several cancer cell lines. The object of this study was to investigate the effects of ginsenoside 20(S)-protopanaxadiol on the invasion in vitro and the expression of matrix metalloprotemase-2 in human fibrosarcoma HT1080 cells in absence of cytotoxicity. Our results showed that ginsenoside 20(S)-protopanaxadiol exerted a concentration-dependent inhibitory effect on the proliferation of HT1080 Cells (IC50 was 76.78 +/- 2.24 mu M, 48 hr). Treatment with 20(S)-protopanaxadiol significantly declined the invasive capacity of HT1080 cells compared to the control cells (P < 0.01) in the in vitro invasion assay. Further analysis with gelatin zymography and western blotting revealed that both the activity and the expression of matrix metalloprotemase-2 decreased dramatically in a concentration-dependent manner (P < 0.01). Taken together, these results indicated that ginsenoside 20(S)-protopanaxadiol is able to inhibit the invasiveness of HT1080 cells significantly in vitro and this action may be primarily due to down-regulating the expression of matrix metalloproteinase-2. Ginsenoside 20(S)-protopanaxadiol, a metabolite of ginseng, may be applied as a potential therapeutic agent in the prevention and treatment of cancer.