Kinetics of in vitro adsorption and entry of papillomavirus virions
Kinetics of in vitro adsorption and entry of papillomavirus virions
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DOI：
10.1016/j.virol.2003.11.004
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发表时间：
2004-02-05
期刊：
影响因子：
3.7
通讯作者：
Christensen, ND
中科院分区：
文献类型：
--
作者：
Culp, TD;Christensen, ND
关于乳头状瘤病毒进入培养细胞的速率的报道有很多不一致之处。我们使用最近开发的QRT-PCR测定法（J. Virol.方法111（2003）135）分析人乳头瘤病毒11型（HPV-11）在多种细胞系中的表达、吸附和进入动力学。还使用生物学相关株系进行了HPV-40和棉尾兔乳头瘤病毒（CRPV）的平行实验。感染是通过表达早期转录本E1布尔ANDE 4剪接点。结果支持先前的观察结果，即乳头瘤病毒进入培养细胞的速度可能比类似结构的病毒报道的速度慢得多（Virology 207（1995）136; Virology 307（2003）1; J. Virol. 75（2001）1565）。此外，我们的数据表明，在吸附到细胞表面后，衣壳结构在很大程度上保持不变，因为HPV-11病毒体对非特异性杀微生物剂和L1特异性单克隆抗体（MAb）的中和作用同样敏感，靶向线性和构象敏感表位。(C)2003年爱思唯尔公司All rights reserved.
There has been much incongruence in reports addressing the rate at which papillomaviruses enter cultured cells. We used a recently developed QRT-PCR assay (J. Virol. Methods 111 (2003) 135) to analyze the expression, adsorption, and entry kinetics of human papillomavirus type 11 (HPV-11) in multiple cell lines. Parallel experiments with HPV-40 and cottontail rabbit papillomavirus (CRPV) were also performed with biologically relevant lines. Infection was determined by the expression of early transcripts containing the E1boolean ANDE4 splice junction. Results support previous observations that papillomaviruses may enter cultured cells much more slowly than rates reported for similarly structured viruses (Virology 207 (1995) 136; Virology 307 (2003) 1; J. Virol. 75 (2001) 1565). Additionally, our data suggest that, following adsorption to the cell surface, capsomeric structure remains largely unchanged for many hours as HPV-11 virions remain equally susceptible to neutralization by a nonspecific microbicide and by L1-specific monoclonal antibodies (MAb) targeting both linear and conformationally sensitive epitopes. (C) 2003 Elsevier Inc. All rights reserved.