Enhancing of measles virus infection by magnetofection
Enhancing of measles virus infection by magnetofection
复制标题
DOI：
10.1016/j.jviromet.2005.04.003
复制
发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Nagata, K
中科院分区：
文献类型：
--
作者：
Kadota, S;Kanayama, T;Nagata, K
磁转染是在磁场下使用聚乙烯亚胺缀合的超顺磁性纳米颗粒的病毒和非病毒基因递送方法。以往的研究表明，磁转染增强了腺病毒和逆转录病毒的感染。它表明，磁转染增强麻疹病毒，副粘病毒的感染。当用编码绿色荧光蛋白基因的麻疹病毒感染表达麻疹病毒受体人SLAM的细胞时，磁转染使麻疹病毒感染增强30至70倍。磁转染也能增强麻疹病毒对SLAM阴性细胞的感染，但程度较低。这些结果表明，磁转染可用于从临床标本中分离麻疹病毒。(c)2005 Elsevier B.V.保留所有权利。
Magnetofection is a viral and non-viral gene delivery method using polyethyleneimine-conjugated super-paramagnetic nanoparticle under a magnetic field. Previous studies have indicated that magnetofection enhanced the infection of adenoviruses and retroviruses. It is shown that magnetofection enhances the infection of measles virus, a paramyxovirus. When cells expressing a measles virus receptor human SLAM were infected with a measles virus that encodes green fluorescent protein gene, magnetofection enhanced measles virus infection by 30- to 70-fold. The infection of SLAM-negative cells with measles virus was also enhanced by magnetofection, but to a lesser extent. These results indicate that magnetofection could be useful for isolation of measles virus from clinical specimens. (c) 2005 Elsevier B.V. All rights reserved.