Antimicrobial Activity of Natural Plant Compound Carvacrol Against Soft Rot Disease Agent Dickeya zeae
Antimicrobial Activity of Natural Plant Compound Carvacrol Against Soft Rot Disease Agent Dickeya zeae
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DOI：
10.1007/s00284-021-02609-3
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发表时间：
2021-07-14
影响因子：
2.6
通讯作者：
Lin, Birun
中科院分区：
文献类型：
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作者：
Jiang, Shangbo;Zhang, Jingxin;Lin, Birun
玉米狄克氏菌是一种全球性的重要细菌病原体，据报道，其在几种基本粮食作物（包括香蕉、水稻、玉米和马铃薯）中引起严重的软腐病。香芹酚是一种疏水性萜烯成分，存在于唇形科芳香植物和各种精油中。然而，关于其对D.玉米。研究香芹酚对D.玉米。测定了香芹酚对D. zeae的最低浓度分别为0.1 mg/mL和0.2 mg/mL。香芹酚对D.玉米等植物细胞内ATP浓度降低、核酸渗漏和膜电位降低。扫描电子显微镜（SEM）显微照片证实，D。香芹酚对玉米细胞膜有损伤作用。此外，D.在用亚抑制浓度的香芹酚处理后，观察到玉米游动运动性和生物膜形成，这表明对这些毒力因子有显著的负面影响。因此，组织感染试验表明香芹酚显著降低了D.玉米。盆栽试验表明，香芹酚处理后香蕉幼苗的症状明显减轻，接种后14 d对香蕉软腐病的防效为32.0%。香芹酚对D.在防治玉米圆斑螟上具有很大的应用潜力。与玉米相关的作物病害。
Dickeya zeae is a globally important bacterial pathogen that has been reported to cause severe soft rot diseases in several essential food crops, including bananas, rice, maize, and potatoes. Carvacrol, a hydrophobic terpene component, is found in aromatic plants of the Labiatae family and various essential oils. However, little work has been done on its antimicrobial potential against D. zeae. This study aimed to evaluate the antimicrobial activity and the functional mechanism of carvacrol against D. zeae. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of carvacrol against D. zeae were 0.1 mg/mL and 0.2 mg/mL, respectively. Carvacrol affected the cell membrane of D. zeae, as revealed by decreased intracellular ATP concentration, nucleic acid leakage, and decreased membrane potential. Scanning electron microscopy (SEM) micrographs confirmed that D. zeae cell membranes were damaged by carvacrol. Furthermore, a significant inhibition of D. zeae swimming motility and biofilm formation was observed following treatments with carvacrol at sub-inhibitory concentrations, indicating a significantly negative effect on these virulence factors. Accordingly, the tissue infection test revealed that carvacrol significantly reduced the pathogenicity of D. zeae. In a pot experiment, inoculated banana seedlings displayed remarkably lesser disease symptoms following treatment with carvacrol, and the control efficiency for banana soft rot was 32.0% at 14 days post-inoculation. To summarize, carvacrol exhibits strong antimicrobial activity against D. zeae and great potential applications in the control of D. zeae-associated crop diseases.