DIFFERENTIAL REGULATION OF THE 2'3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE GENE DURING OLIGODENDROCYTE DEVELOPMENT
DIFFERENTIAL REGULATION OF THE 2'3'-CYCLIC NUCLEOTIDE 3'-PHOSPHODIESTERASE GENE DURING OLIGODENDROCYTE DEVELOPMENT
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DOI：
10.1016/0896-6273(94)90451-0
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发表时间：
1994-06-01
期刊：
影响因子：
16.2
通讯作者：
KAMHOLZ, J
中科院分区：
文献类型：
--
作者：
SCHERER, SS;BRAUN, PE;KAMHOLZ, J
最近已证明 2',3'-环核苷酸磷酸二酯酶 (CNP) 的两种主要亚型（48 和 46 kDa）是通过单个基因通过选择性剪接产生的。此外，编码较大亚型的信使RNA是从一个单独的启动子转录的，该启动子位于编码较小亚型的启动子上游约1 kb处。我们研究了这两种CNP亚型的表达，发现它们在少突胶质细胞成熟过程中存在差异表达。在少突胶质细胞前体中，仅发现编码较大蛋白质的 mRNA。然而，在少突胶质细胞分化时，两种 CNP mRNA 都会被诱导。这些 CNP 表达模式可能是由于少突胶质细胞分化过程中两个 CNP 启动子的阶段特异性转录调控所致。
The two major isoforms of 2',3'-cyclic nucleotide phosphodiesterase (CNP), 48 and 46 kDa, have recently been shown to be produced from a single gene by alternative splicing. In addition, messenger RNA encoding the larger isoform is transcribed from a separate promoter, approximately 1 kb upstream from that encoding the smaller isoform. We have investigated the expression of these two CNP isoforms and have found that they are differentially expressed during the process of oligodendrocyte maturation. In oligodendrocyte precursors, only the mRNA encoding the larger protein is found. At the time of oligodendrocyte differentiation, however, both CNP mRNAs are induced. These patterns of CNP expression are likely due to stage-specific transcriptional regulation of the two CNP promoters during the process of oligodendrocyte differentiation.