Direct coupling between meiotic DNA replication and recombination initiation
Direct coupling between meiotic DNA replication and recombination initiation
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DOI：
10.1126/science.290.5492.806
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发表时间：
2000-10-27
期刊：
影响因子：
56.9
通讯作者：
Lichten, M
中科院分区：
文献类型：
--
作者：
Borde, V;Goldman, ASH;Lichten, M
在酿酒酵母减数分裂期间，DNA 复制发生在 DNA 双链断裂形成重组开始之前 1.5 至 2 小时。我们证明复制和重组起始是直接相关的。阻断减数分裂复制以独立于复制检查点的方式防止双链断裂形成，并延迟染色体片段的复制，特别是延迟该片段中断裂的形成。因此，复制和断裂形成之间的时间在所有区域中保持恒定。我们认为，双链断裂的形成是 DNA 复制启动过程的一部分，因此决定了减数分裂重组何时在区域而非细胞范围内启动。
During meiosis in Saccharomyces cerevisiae, DNA replication occurs 1.5 to 2 hours before recombination initiates by DNA double-strand break formation. We show that replication and recombination initiation are directly linked. Blocking meiotic replication prevented double-strand break formation in a replication-checkpoint-independent manner, and delaying replication of a chromosome segment specifically delayed break formation in that segment. Consequently, the time between replication and break formation was held constant in all regions. We suggest that double-strand break formation occurs as part of a process initiated by DNA replication, which thus determines when meiotic recombination initiates on a regional rather than a cell-wide basis.